Spheroid Microplates

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Ready to get started in 3D cell culture? You’ve come to the right place. 

3D Spheroids Simplified with Corning

Generate, culture, assay, and analyze your spheroids all in one microplate. The Corning® spheroid microplate features a novel and proprietary design that allows you to use one microplate to achieve all of these steps without manipulating or transferring delicate spheroids. The microplates feature black opaque walls and clear, round well-bottom geometry, as well as the Corning Ultra-Low Attachment surface, which is hydrophilic, biologically inert and non-degradable. All Corning spheroid microplates feature a unique well-bottom design that enables highly reproducible growth of 3D spheroid cultures. The opaque side walls and gridded bottom design reduce well-to-well cross-talk and background fluorescence/luminescence.

The Corning spheroid microplate is available in 96-, 384-, and 1536-well formats. The 96-well Corning spheroid microplate is compatible with the HTS Transwell®-96 tissue culture system for 3D immune oncology models for high throughput testing.

The addition of the 1536-well format allows customers to work with Corning products throughout the whole drug discovery workflow. The higher density format enables 3D cell culture in a HTS environment. Each microplate is compatible with most automated imagers.

Features & Benefits

ULA Surface and Common Applications

Corning Ultra-Low Attachment Surface - Featured on Spheroid Microplates

Corning spheroid microplates are coated with an Ultra-Low Attachment (ULA) surface, which has a covalently bonded hydrogel designed to minimize cell attachment, protein absorption, enzyme activation, and cellular activation. This surface is noncytotoxic, biologically inert, and nondegradable. The non-ionic hydrogel layer reduces or eliminates ionic and hydrophobic binding which prevents or reduces cell attachment and binding.

Ultra-Low Attachment surface is most appropriate for assays where preventing cell attachment is required or in hybridoma production and clonal isolation by limiting dilution.

Spheroid Microplate Guidelines for Use

Data shows drastic differences in cell behavior and response to drugs when cells are cultured in 3D compared to 2D models. However, 3D systems come with some challenges in terms of optimization, handling, and assaying. Review our considerations for Three-Dimensional Cell Culture when using the Corning Spheroid Microplate.

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Corning Spheroid Microplate User Guide

This user guide describes a basic method for generating and culturing multicellular spheroids. Plating volumes and densities are cell line- and downstream application-dependent, therefore assay specific optimization of conditions is recommended.

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Spheroid Formation Protocol

To recapitulate features of native tumor microenvironments, cancer cells can be cultured in Corning spheroid microplates, which combine the Corning Ultra-Low Attachment surface with innovative well geometry to provide an ideal tool for generating, culturing, and assaying 3D multicellular spheroids in the same plate.

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Spheroid Processing and Embedding for Histology

In this guidelines for use, learn a procedure for the fixation of spheroids, followed by transfer to an embedding medium for processing and imaging.

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Using 3D human spheroids as a predictive model in drug discovery

Discover how StemoniX used 3D human spheroids as a predictive model in drug discovery using the Corning spheroid microplate.

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Corning spheroid microplates open up new possibilities in cancer therapy

Learn more about researchers at Uppsala University who used Corning spheroid microplates to scale up spheroids, opening up new possibilities in cancer therapy.

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How to Buy