Ultra-Low Attachment Surface | Enable 3D Spheroid Formation | Corning

Overview

Corning Ultra-Low Attachment (ULA) surface microplate

Enable 3D Spheroid Formation with Corning Ultra-Low Attachment Surface

Enable 3D Spheroid Formation with Corning Ultra-Low Attachment Surface

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Corning Ultra-Low Attachment surface is a hydrophilic, neutrally charged coating covalently bound to a polystyrene vessel surface. The hydrogel inhibits specific and nonspecific immobilization, forcing cells into a suspended state, enabling 3D spheroid formation. The coating is stable, noncytotoxic, biologically inert, and non-degradable—and can be applied to a wide range of cultureware. These include plates, dishes, flasks, and Corning CellSTACK® vessels as well as ultra low attachment 96-well and 384-well plates for high throughput screening (HTS) spheroid applications.

Corning Ultra-Low Attachment surface is a hydrophilic, neutrally charged coating covalently bound to a polystyrene vessel surface. The hydrogel inhibits specific and nonspecific immobilization, forcing cells into a suspended state, enabling 3D spheroid formation. The coating is stable, noncytotoxic, biologically inert, and non-degradable—and can be applied to a wide range of cultureware. These include plates, dishes, flasks, and Corning CellSTACK® vessels as well as ultra low attachment 96-well and 384-well plates for high throughput screening (HTS) spheroid applications.

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Ultra-low Attachment Surface

About

Characteristics and Applications

Formats

Plates: 6-well, 24-well, 96-well flat (clear), 96-well round bottom (black/clear), 384-well flat bottom (black/clear), 384-well round bottom (black/clear).

Dishes: 60 mm, 100 mm

Flasks: T-25, T-75, Corning CellSTACK: 1-layer

Applications

•    Enabling 3D spheroid formation, such as embryoid body and tumorsphere formation

•    Neurobiology for neurosphere formation
•    Embryoid body formation in stem cell biology
•    Suspension cell-based screening
•    Culturing cells, tissues, or organ fragments
•    Studying activation and inactivation mechanisms of lymphocytes, monocytes, macrophages, and other phagocytic cells
•    Spheroid formation in cancer research

 

Surface TechnologyCovalently bound hydrophilic, non-ionic, neutrally charged hydrogel
Features and Benefit

Features and Benefit

Features and Benefit

The Ultra-Low Attachment surface is designed for:

  • Maintaining cells in a suspended, unattached state
  • Preventing stem cells from attachment-mediated differentiation
  • Preventing anchorage-dependent cells from dividing
  • Reducing binding of attachment and serum proteins to the substrate
  • Formation of 3D multicellular spheroids

The Ultra-Low Attachment surface is designed for:

  • Maintaining cells in a suspended, unattached state
  • Preventing stem cells from attachment-mediated differentiation
  • Preventing anchorage-dependent cells from dividing
  • Reducing binding of attachment and serum proteins to the substrate
  • Formation of 3D multicellular spheroids
Surfaces

Corning Surfaces for Cell Culture

Corning Surfaces for Cell Culture

Untreated polystyrene has an uncharged, hydrophobic surface to which cell attachment proteins bind poorly. This results in poor and very uneven cell attachment and growth.

Tissue Culture (TC)-treated polystyrene has a negatively charged, hydrophilic surface to which cell attachment proteins bind evenly. This provides a good surface for cell attachment and growth.

The Ultra-Low Attachment surface has a neutral, hydrophilic hydrogel coating that greatly reduces binding of attachment proteins. This minimizes cell attachment and spreading.

Untreated polystyrene has an uncharged, hydrophobic surface to which cell attachment proteins bind poorly. This results in poor and very uneven cell attachment and growth.

Tissue Culture (TC)-treated polystyrene has a negatively charged, hydrophilic surface to which cell attachment proteins bind evenly. This provides a good surface for cell attachment and growth.

The Ultra-Low Attachment surface has a neutral, hydrophilic hydrogel coating that greatly reduces binding of attachment proteins. This minimizes cell attachment and spreading.

Cells

Cells that Culture Successfully on Corning Ultra-Low Attachment Surface Coating

Primary Cells

Colonocytes (epithelial)

Keratinocytes

Mammary epithelial cells; breast cells (luminal, myoepithelial, and endothelial)

Pancreatic islet, neonatal (3- to 5-day-old) rat islets of Langerhans

Peripheral blood mononuclear cells

Cell Lines (Transformed or Transfected)

C2C12 (myoblast)

HEK-293 (transfected, epithelial), EcoPack2™-293, HEK-SRAtet cells, Living Colors HEK-ZsGreen proteasome sensor (transfected)

HepG2 (hepatocyte)

HT-1080 (epithelial)

hFOB 1.19, MG63 (osteoblast cell lines)

LnCAP (prostate cancer cell line)

MCF7 (epithelial)

MCF-10A (epithelial)

MDA-MB-231

U937 (monocyte)

Stem and Progenitor Cell Expansion

Human embryonic stem cell (hESC)

rESC; Rat Endothelial Progenitor cells

Neuronal Stem Cell

In Vitro Differentiation of Pluripotent Stem Cells

hESC, hiPSC, mESC (Germ Cell Layers: ectoderm, mesoderm, endoderm; hematopoietic progenitor; definitive differentiation; cardiomyocytes)

hESC, hiPSC (intestinal organoids)

hESC, hiPSC (neuronal)

hESC, mESC (lung epithelial)

hESC, mESC, rESC (hepatocyte, hepatocyte-like)

hPSCs, mPSCs (renal progenitor cells, renal tubular cells, endoderm)

mESC, rESC, miPSC (neuronal, progenitor)

In Vitro Differentiation of Adult Stem Cells

hADSCs; Adipose (endothelial)

Cardiac Progenitor Cells (cardiomyocyte)

Colon (epithelial organoids)

Hepatic Progenitor Cells (hepatic, bilary cells)

Lung (sphere)

Mammary epithelial cells

MSC (cardiomyocyte, chondrocyte, hematopoetic, hepatocyte, neuron, osteocyte, spheroid)

MSC (endothelial progenitors)

Neural progenitor/stem cells (neuron, astrocytes, neuroblast)

3D Cell Culture Applications

MCF-7 (epithelial)

MCF-10A (epithelial)

MDA-MB-231

HeLa

HT-1080 (epithelial)

hESC, Rat (endothelium)

MSCs, ovarian cancer cells (OCC)

 

Applications and Use

Ultra-Low Attachment Surface in Action

Surface Selection Guide

Featured Products, Resources, and More

Surface Selection Guide

Surface Selection Guide

Create physiologically relevant in vitro models that support cell growth and function with the right surface from Corning.

Create physiologically relevant in vitro models that support cell growth and function with the right surface from Corning.

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Corning® Ultra-Low Attachment Surface Bibliography

Featured Products, Resources, and More

Corning® Ultra-Low Attachment Surface Bibliography:

Corning® Ultra-Low Attachment Surface Bibliography:

Review a partial bibliography of the stem cell research done using Corning® Ultra-Low Attachment.

Review a partial bibliography of the stem cell research done using Corning® Ultra-Low Attachment.

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Corning® 1536 Spheroid Microplate

Featured Products, Resources, and More

Corning® 1536 Spheroid Microplate

Corning® 1536 Spheroid Microplate

With their novel and proprietary design, these microplates are ideal for generating and analyzing 3D multicellular spheroids in the same microplate.

With their novel and proprietary design, these microplates are ideal for generating and analyzing 3D multicellular spheroids in the same microplate.

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How to Buy

Purchasing Options

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It’s easy to purchase Ultra-Low Attachment surface-coated vessels online. Just browse our online catalog, and add products directly to your cart. Order direct with a credit card or use a purchase order if you have an account with Corning.

It’s easy to purchase Ultra-Low Attachment surface-coated vessels online. Just browse our online catalog, and add products directly to your cart. Order direct with a credit card or use a purchase order if you have an account with Corning.

Order Now
Distributors

Distributors

Distributors

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Contact your authorized distributor to order Ultra-Low Attachment surface-coated vessels.

Contact your authorized distributor to order Ultra-Low Attachment surface-coated vessels.

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Customer Service

Customer Service

Customer Service

Contact Us

If you need assistance selecting products, or if you’d like to order by phone, fax, or email, please contact us.

If you need assistance selecting products, or if you’d like to order by phone, fax, or email, please contact us.

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Related Products

Related Products

Corning 96 and 384 Well Spheroid Microplates

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Now you can generate and analyze 3D multicellular spheroids in the same microplate, thanks to the innovative design of Corning Ultra-low Attachment 96 Well and 384 Well microplates. The Ultra-Low Attachment surface enables uniform and reproducible spheroid formation.

Now you can generate and analyze 3D multicellular spheroids in the same microplate, thanks to the innovative design of Corning Ultra-low Attachment 96 Well and 384 Well microplates. The Ultra-Low Attachment surface enables uniform and reproducible spheroid formation.

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CellSTACK Chamber

Ultra-low Attachment Surface CellSTACK Chamber Ultra-low Attachment Surface CellSTACK Chamber

Scale-up your non-adherent cell culture with Corning CellSTACK chambers coated with Ultra-low Attachment surface.

Scale-up your non-adherent cell culture with Corning CellSTACK chambers coated with Ultra-low Attachment surface.

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