Matrigel Matrix | Extracellular Matrix | Corning

Overview

Corning Matrigel Matrix

Cells behave better on Corning Matrigel matrix—the original, trusted extracellular matrix (ECM).

Nearly 30 years ago, researchers were looking for a way to grow mouse sarcoma cells. The solution? The development of Corning Matrigel matrix. Matrigel is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in such ECM proteins as laminin (a major component), collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Today, this natural ECM-based hydrogel is among the most widely used models for 2D and 3D cell culture in vitro, enabling you to:

 

  • Improve the attachment and differentiation of both normal and transformed anchorage-dependent epithelioid cells, as well as other cell types.
  • Better mimic in vivo environments for 2D and 3D cell culture applications.
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SPECIAL OFFER: For purchases April 15, 2023 through October 15, 2023

Buy 15 vials or more of select Corning Matrigel matrix products, get 1 case of select cell culture product AND 1 case of select liquid handling product FREE

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SPECIAL OFFER: For purchases April 15, 2023 through October 15, 2023

Buy 15 vials or more of select Corning Matrigel matrix products, get 1 case of select cell culture product AND 1 case of select liquid handling product FREE

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About
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Characteristics of Matrigel Matrix
 Standard FormulationHigh Concentration (HC)Growth Factor Reduced (GFR)Phenol Red Free

hESC-qualified

Application

Suitable for culture of polarized cells, such as epithelial cells.

Promotes differentiation of many cell types, including hepatocytes, neurons, beta-islets, mammary epithelial, endothelial, and smooth muscle cells.

Higher protein concentration provides greater matrix stiffness and scaffold integrity.

Suitable for in vivo cell delivery applications for improved cell engraftment and augmentation of solid tumor formation.

Suited for applications where a more highly defined basement membrane preparation is desired.

Available in standard, Phenol red-free, and GFR formulations.

Suitable for assays that require color detection (e.g., colorimetric, fluorescence).Available in standard, GFR, and HC formulations.Pre-screened for compatibility with mTeSR®1 medium by Stem Cell Technologies, providing the reproducibility and consistency essential for human embryonic and induced pluripotent feeder-free stem cell culture.

Source

Mouse

Mouse

Mouse

Mouse

Mouse

Protein Concentration*

8-12 mg/mL

 

18-22 mg/mL

 

8-12 mg/mL

 

8-12 mg/mL

 

Dilution Factor

 

Shelf Life

2 years from date of manufacture.

Date of expiration is located on a lot-specific certificate of analysis.

2 years from date of manufacture.

Date of expiration is located on a lot-specific certificate of analysis.

 

2 years from date of manufacture.

Date of expiration is located on a lot-specific certificate of analysis.

 

2 years from date of manufacture.

Date of expiration is located on a lot-specific certificate of analysis.

 

2 years from date of manufacture.

Date of expiration is located on a lot-specific certificate of analysis.

 

Vialed Formats

(Cat. No./Qty.)

356234 | 5 mL

354234 | 10 mL

356235 | 5 x 10 mL

356237 | 10 mL (Phenol red-free)

354248 | 10 mL

354262 | 10 mL (Phenol red-free)

354263 | 10 mL (GFR)

356230 | 5 mL (Standard)

354230 | 10 mL (Standard)

354263 | 10 mL (HC)

356231 | 10 mL (Phenol red-free)

356237 | 10 mL (Standard)

354262 | 10 mL (HC)

356231 | 10 mL (GFR)

354277 | 5 mL

 

BioCoatTM Options

Plates: 6 well, 12 well, 24 well, 48 well, 96 well

Inserts: for 24 well plates

Dishes: 35 mm, 60 mm, 100 mm

    

*Protein concentration mentioned above are typical ranges; in some instances, lots may fall outside this range.
See Certificate of analysis for information on individual lots.

Features

Unique features of Matrigel matrix include:

Unique features of Matrigel matrix include:

  • Proven and trusted since 1985; most extensively referenced basement membrane matrix.
  • Wide selection of basement membranes; used in diverse applications, from cancer and stem cell research to neurobiology.
  • Manufactured under stringent quality control practices that exceed industry standards, ensuring protein consistency and superior product performance.
  • Rigorous validation for functionality.
  • Certified LDEV-free.
  • Proven and trusted since 1985; most extensively referenced basement membrane matrix.
  • Wide selection of basement membranes; used in diverse applications, from cancer and stem cell research to neurobiology.
  • Manufactured under stringent quality control practices that exceed industry standards, ensuring protein consistency and superior product performance.
  • Rigorous validation for functionality.
  • Certified LDEV-free.
Cells
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Compatible Cell Types

Primary Cells

 

Cell Lines (Transformed or Transfected)

Stem and Progenitor Cell Expansion

In Vitro Differentiation of Pluripotent Stem Cells

In Vitro Differentiation of Adult Stem Cells

Aortic endothelial cells, BAEC

Bile duct cells (epithelial)

Brain microvessel (endothelial)

Cardiomyocytes; cardiac (endothelium, progenitor cells)

Colonocytes (epithelial)

Dorsal root ganglia

Embryonic cortical neurons

Embryonic sympathetic neurons

Hepatocytes

Hippocampal neurons

HUVEC (endothelial)

HVSMC

Keratinocytes

Mammary epithelial cells; breast cells (luminal, myoepithelial and endothelial)

Microvascular, BME (endothelial)

Mouse splenic T-Cells

Muscle cells, myoblasts, myogenic cells, myotubes

Oligodendrocytes (glial; precursors)

Pancreatic islet, neonatal (3- to 5-day-old) rat islets of Langerhans

Parotid acinar cells

Peripheral blood mononuclear cells

Schwann cells (glial)

Sertoli cells (spermogenic)

Skeletal muscle cells (myocytes, myotubes)

Smooth muscle cells (endothelial, vascular)

Urothelial cells

Breast cancer cells (established cell lines)

C2C12 (myoblast)

CHO, CHO-1, CHO-K1 (epithelial, endothelial, transfected fusion protein)

COS-7 (fibroblast, transfected)

Dorsal Root Ganglia (transfected)

H1299 (transfected- human non-small cell lung carcinoma cell line)

HEK-293 (transfected, epithelial), EcoPack2™-293, HEK-SRAtet cells, Living Colors HEK-ZsGreen proteasome sensor (transfected)

HepG2 (hepatocyte)

HT-1080 (epithelial)

hFOB 1.19, MG63 (osteoblast cell lines)

L929 (fibroblast, transfected)

LnCAP (prostate cancer cell line)

MCF-10A (epithelial

MDA-MB-231

MDA-MB 435

NIH/3T3, 3T3 (fibroblast)

PC-3, PC-12

RTG-2 (rainbow trout gonad cells)

SH-SY5Y

 

Human embryonic stem cell (hESC)

Human induced pluripotent stem cell (hiPSC)

 

hESC (cerebral organoid model)

hESC (pancreatic)

hESC, hiPSC (cardiomyocytes)

hESC, hiPSC, mESC (Germ Cell Layers: ectoderm, mesoderm, endoderm; hematopoietic progenitor; definitive differentiation; cardiomyocytes)

hESC, hiPSC, mESC, miPSC (endothelial)

hESC, hiPSC (intestinal organoids)

hESC, hiPSC (neuronal)

hESC, hiPSC (smooth muscle)

hESC, mESC (lung epithelial)

hESC, mESC, rESC (hepatocyte, hepatocyte-like)

Human NPCs (differentiation to neuronal cells)

hPSCs, mPSCs (renal progenitor cells, renal tubular cells, endoderm)

mESC (hematopoietic)

mESC, Chicken (cardiomyocytes)

mESC, rESC, miPSC (neuronal, progenitor)

mPSCs (inner ear sensory epithelia)

 

hADSCs; Adipose (endothelial)

Colon (epithelial organoids)

Hair Follicle (melanocytes, neurons, smooth muscle)

Intestinal (organoids, crypt-villus)

 

Applications

Corning Matrigel matrix is effective for the attachment and differentiation of both normal and transformed anchorage-dependent epithelioid and other cell types. These include neurons, Sertoli cells, chick lens, vascular endothelial cells, and hepatocytes.

Applications include:


  • Influencing gene expression in adult rat hepatocytes
  • 3D culture in mouse and human mammary epithelial cells
  • In vivo peripheral nerve regeneration
  • Metabolism and toxicology studies
  • The development of several types of tumor cell invasion assays
  • The in vitro and in vivo study of angiogenesis
  • In vivo propagation of human tumors in immunosuppressed mice
  • The maintenance of human embryonic stem cells and induced pluripotent stem (hiPS) cells
  • Neuronal differentiation of hiPS cells

Matrigel Matrix Quality Control Specifications

  • Mouse colonies routinely screened for pathogens via mouse antibody production (MAP) testing
  • LDEV-free EHS tumor
  • Extensive PCR testing performed on a number of pathogens, including LDEV, ensuring strict control of raw materials used during manufacturing
  • Tested and found negative for the presence of bacteria, fungi, and mycoplasma
  • Protein concentrations determined by Lowry method
  • Endotoxin units measured by Limulus Amoebocyte Lysate assay
  • Gel stability tested for a period of 14 days at 37°C
  • Biological activity determined for each lot using a neurite outgrowth assay; chick dorsal root ganglia, plated on a 1.0 mm layer of Corning Matrigel matrix, required to generate positive neurite outgrowth response after 48 hours without addition of nerve growth factor

Free Download

Request a copy of the Ultimate Guide to Matrigel Matrix – a go-to resource packed with a plethora of resources from top tips and facts to expert advice on Matrigel Matrix.

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Applications and Use

Matrigel Matrix in Action

Featured Resources

FAQ

Corning Matrigel Matrix FAQs

From growth factor concentration to cell recovery and storage, learn how to optimize your cell culture with Corning Matrigel matrix.

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Corning Matrigel Lot Selection and Reserve Tool

Corning Matrigel Lot Selection and Reserve Tool

Make sure your experiments are as consistent as possible. Reserve a consistent supply of Matrigel.

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Infographic: Why is Corning Matrigel Matrix so Popular?

Infographic: Why is Corning Matrigel Matrix so Popular?

Corning Matrigel matrix is the most highly referenced extracellular matrix and it's widely used for 2D and 3D studies, with 13,000+ citations.

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Basics of Corning® Matrigel® Matrix

Basics of Corning® Matrigel® Matrix

This webinar introduces basic information on Corning Matrigel matrix and discuss tips and techniques for handling Corning Matrigel matrix.

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Choosing the Right Cell Culture Surface: Modified Surfaces

Choosing the Right Cell Culture Surface: Modified Surfaces

In this webinar you will learn basic principles behind tissue culture treatment, and when to use enhanced attachment surfaces.

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Choosing the Right Cell Culture Surface: Extracellular Matrix Coatings

Choosing the Right Cell Culture Surface: Extracellular Matrix Coatings

In this webinar you will learn key differences between natural, mimetic, and synthetic ECM coatings and applications for specific ECM coatings.

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Why HUB Organoids are Key to Advancing Personalized Cancer Therapies

Why HUB Organoids are Key to Advancing Personalized Cancer Therapies

HUB Organoids details how patient-derived organoids are becoming promising models for personalized treatment using 3D cell culture technologies from Corning & Yamaha Corporation.

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Using Organoids in the Study of Infectious Diseases

Using Organoids in the Study of Infectious Diseases

Organoid cell culture has transformed cell-based assays in drug discovery and basic biology by conferring physiologic relevance to in vitro cell-based biological models.

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A Life Scientist’s How-To Guide on Corning Matrigel Matrix and Organoids

A Life Scientist’s How-To Guide on Corning Matrigel Matrix and Organoids

This guide details best practices to consider for organoid work, as well as how to use Corning Matrigel matrix and other products to support your organ-specific research.

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Guide to 3D Model Systems: Spheroids, Organoids and Tissue Models

Guide to 3D Model Systems: Spheroids, Organoids and Tissue Models

Download this guide to 3D models with helpful tips & techniques to set your 3D culture up for success.

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Application Notes

Tuning the Elastic Moduli of Corning® Matrigel® and Collagen I 3D Matrices by Varying the Protein Concentration

Tuning the Elastic Moduli of Corning® Matrigel® and Collagen I 3D Matrices by Varying the Protein Concentration

In this paper, we investigated the effect of protein concentration on the elastic moduli of Corning Matrigel® matrix and Collagen I gels using a rotational rheometer.

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Protocol for Corning® Matrigel® Basement Membrane Matrix for 3D Culture In Vitro

Protocol for Corning® Matrigel® Basement Membrane Matrix for 3D Culture In Vitro

Step-by-step protocol for on-top MDCK 3D culture and embedded MDCK 3D culture.

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A Novel Method for Generating Single, Intestinal Organoids for High Throughput Screening

A Novel Method for Generating Single, Intestinal Organoids for High Throughput Screening

Here we demonstrate the formation of gastrointestinal organoids derived from human induced pluripotent stem cells (hiPSC) in Corning® 96-well spheroid microplates in combination with Corning Matrigel® matrix, resulting in a single intestinal organoid in each well.

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Methods for Implantation of Corning Matrigel Matrix into Mice and Tissue Fixation

Methods for Implantation of Corning Matrigel Matrix into Mice and Tissue Fixation

Use Matrigel matrix in angiogenesis and human tumor implantation in mice.

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Maintenance of Human iPS Cells in a Feeder-free Culture System

Maintenance of Human iPS Cells in a Feeder-free Culture System

Corning Matrigel matrix is an effective surface that supports feeder-free expansion and maintenance of undifferentiated human iPS cells.

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Corning Matrigel Matrix

View All Matrigel Application Notes

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How to Buy

Purchasing Options

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It’s easy to purchase Matrigel matrix online. Order direct with a credit card or use a purchase order if you have an account with Corning.

Distributors

Distributors

Contact your authorized distributor to order Matrigel matrix.

Contact your authorized distributor to order Matrigel matrix.

Customer Service

Customer Service

If you need assistance selecting products, or if you’d like to order by phone, fax, or email, please contact us.

If you need assistance selecting products, or if you’d like to order by phone, fax, or email, please contact us.

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