The Corning X-MINI® Selection Kit is designed for the selection of cells and cell subsets from blood and blood products through Buoyancy Activated Cell Sorting (X-BACS) technology, which employs microbubbles to isolate target cells in a semi-automated, functionally closed system. The X-MINI selection kit provides a highly-purified preparation of target cells, with a high recovery efficiency and cell viability. Isolate cells quickly and easily from a variety of sources such as, PMBC, dissociated tissues, whole blood, buffy coats, and more.
Overview
Isolate Cells with X-BACS Technology
Isolate Cells with X-BACS Technology
The Corning® X-SERIES® Systems are manufactured for Corning by ThermoGenesis Corp.
X-SERIES®, X-WASH®, X-LAB®, X-BACS™, and X-MINI® are either registered trademarks or trademarks of ThermoGenesis Corp. in the United States and/or other countries.
About
About the X-MINI Selection Kit
Microbubble Characteristics
Microbubble Characteristics
- The Corning X-BACS Reagent is made up of lipid-based, streptavidin-coated microbubbles that are gas filled and exhibit buoyancy when reconstituted
- When paired with a biotinylated antibody, cell selection can occur by floating selected cells to the surface during centrifugation while pelleting unwanted cells
- The microbubbles are tailor made and fine-tuned for cell separation
- Unlike other selection platforms, there is no toxic exposure to iron from other bead-based systems
- Isolated cells are highly purified and ready for downstream use
- The Corning X-BACS Reagent is made up of lipid-based, streptavidin-coated microbubbles that are gas filled and exhibit buoyancy when reconstituted
- When paired with a biotinylated antibody, cell selection can occur by floating selected cells to the surface during centrifugation while pelleting unwanted cells
- The microbubbles are tailor made and fine-tuned for cell separation
- Unlike other selection platforms, there is no toxic exposure to iron from other bead-based systems
- Isolated cells are highly purified and ready for downstream use
The X-BACS Advantage
The X-BACS Advantage
- Magnet-free–no need to buy additional expensive components
- Gentle on cells–cells are gently floated for separation
- Column-free–the cells are carried and separated by microbubbles directly in the vessel
- Consistent–obtain viable, functional cells with reproducible results
- Magnet-free–no need to buy additional expensive components
- Gentle on cells–cells are gently floated for separation
- Column-free–the cells are carried and separated by microbubbles directly in the vessel
- Consistent–obtain viable, functional cells with reproducible results
Components of the X-MINI Selection Kit
Components of the X-MINI Selection Kit
The X-MINI Selection Kit includes:
- Biotinylated Antibody
- X-BACS Buffer
- X-BACS Reagent
- Syringe
- Needles
- Caps
X-MINI Selection Kit Accessories
- X-MINI Pressor
The X-MINI Selection Kit includes:
- Biotinylated Antibody
- X-BACS Buffer
- X-BACS Reagent
- Syringe
- Needles
- Caps
X-MINI Selection Kit Accessories
- X-MINI Pressor
Users start with blood or blood products such as whole blood, bone marrow, cord blood or MNC (mononuclear cell) fraction derived from the blood product. A biotinylated antibody to the antigen on the surface of the user’s desired cell type (positive selection) or to the antigens on the surface of the unwanted cell types (negative selection) is added to the cell suspension and briefly incubated to attach the antibody to the target cells.
Next, X-BACS Reagent containing streptavidin-coated lipid microbubbles are added to the solution; the microbubbles attach to the biotinylated antibody-targeted cells during another brief incubation. Once the microbubbles and targets cells are attached, the cell mixture is centrifuged to separate the unwanted/non-target cells: the gas-filled buoyant microbubble-linked target cells rise to the top of the solution, while the non-target cells concentrate at the bottom of the vessel.
The user then removes the microbubble selected cell fraction from the container and degases the microbubbles to eliminate buoyancy either manually or using Corning’s X-MINI Pressor. Further wash, activation, or other manipulation steps may be performed at the user’s discretion.
Users start with blood or blood products such as whole blood, bone marrow, cord blood or MNC (mononuclear cell) fraction derived from the blood product. A biotinylated antibody to the antigen on the surface of the user’s desired cell type (positive selection) or to the antigens on the surface of the unwanted cell types (negative selection) is added to the cell suspension and briefly incubated to attach the antibody to the target cells.
Next, X-BACS Reagent containing streptavidin-coated lipid microbubbles are added to the solution; the microbubbles attach to the biotinylated antibody-targeted cells during another brief incubation. Once the microbubbles and targets cells are attached, the cell mixture is centrifuged to separate the unwanted/non-target cells: the gas-filled buoyant microbubble-linked target cells rise to the top of the solution, while the non-target cells concentrate at the bottom of the vessel.
The user then removes the microbubble selected cell fraction from the container and degases the microbubbles to eliminate buoyancy either manually or using Corning’s X-MINI Pressor. Further wash, activation, or other manipulation steps may be performed at the user’s discretion.
Applications
Research Applications for the X-BACS Technology
Resources
Featured Resources
How to Buy
Purchasing Options
Order Now
It’s easy to purchase the X-MINI Selection Kit. Browse our online catalog, and add products directly to your cart. Order direct with a credit card or use a purchase order if you have an account with Corning.
It’s easy to purchase the X-MINI Selection Kit. Browse our online catalog, and add products directly to your cart. Order direct with a credit card or use a purchase order if you have an account with Corning.
Contact Us
If you need assistance purchasing products from the X-MINI Selection Kit, or if you’d like to order by phone, fax, or email, please contact us.
If you need assistance purchasing products from the X-MINI Selection Kit, or if you’d like to order by phone, fax, or email, please contact us.
Learn More
Many distributors offer products from the X-MINI Selection Kit.
Many distributors offer products from the X-MINI Selection Kit.
Related Products
You may also be interested in these related products.
