With the effectiveness of 3D cell culture in many areas of research, including anti-cancer drug screening and in vitro tumor studies, the need for better methods to produce replicate spheroids of uniform size in mass quantities has emerged. The Corning Elplasia 48K vessel addresses this need by enabling researchers to generate a high density of spheroids in a scaffold-free model.
The Corning Elplasia 48K vessel has a 320 cm2 surface area with 152 microcavities per cm2 generates approximately 48,000 spheroids of uniform size and shape per vessel (4X the output of the Elplasia 12K flask and open well plate). Gravity, in conjunction with the Corning Ultra-Low Attachment (ULA) surface, and a rounded microcavity geometry enable formation of approximately 48,000 spheroids of similar shape and size.
The ULA surface, a proprietary, animal-free, covalently bonded hydrogel, promotes the formation and easy harvesting of spheroids. The microcavity geometry allows spheroids to remain in place during medium exchange without compromising full recovery at harvest time. The vessel’s internal diverter feature allows for minimal disruption of spheroids during liquid handling steps.
The Corning Elplasia 48K vessel is compatible with many tumor, normal, and primary cell types often used for 3D cell culture, and may be used across many applications including:
- Spheroid biobanking
- Drug screening
- Cancer/Tumor biology
- Stem cell biology
- Regenerative medicine and cell therapy research
- Extracellular Vesicle (EV) production
