General Guide for Cryogenically Storing Animal Cell Cultures | Corning

Your cells are precious. You have enough variables to manage without having to worry about whether you’re maintaining proper temperature at every stage of the cell culture process.

Ensure standardized, controlled-rate freezing for all cell types with Corning cryogenic storage.

That’s why we created this comprehensive guide to help you successfully freeze animal cells and manage a cell repository. This guide will give you practical advice on:

  • Advantages of freezing cell cultures
  • Cell temperature protocols
  • General events during cell freezing
  • Practical aspects of cell freezing including cell harvesting, cryoprotection, and thawing and recovery

Importance of Cryopreservation

Cryopreservation puts cells in suspended animation protecting cells from damage or contamination from bacteria, fungi, mycoplasma, and yeast. This process safeguards cell integrity for multiple applications in investigative research (like biobanking) and theapeutic use (like regenerative or reporductive medicine). 

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