With the effectiveness of 3D cell culture in many areas of research, including anti-cancer drug screening and in vitro tumor studies, the need for better methods to produce replicate spheroids of uniform size in mass quantities has emerged. The Corning Elplasia 12K flask addresses this need by enabling researchers to generate a high density of spheroids in a scaffold-free model.
The Corning Elplasia 12K flask contains 152 microcavities per cm2 in a vessel footprint similar to that of a T-75 flask. Gravity, in conjunction with the Corning Ultra-Low Attachment (ULA) surface, and a rounded microcavity geometry enable formation of approximately 12,000 spheroids of similar shape and size.
The ULA surface, a proprietary, animal-free, covalently bonded hydrogel, promotes the formation and easy harvesting of spheroids.The microcavity geometry allows spheroids to remain in place during medium exchange without compromising full recovery at harvest time. The flask’s internal diverter feature allows for minimal disruption of spheroids during liquid handling steps.
The Corning Elplasia 12K flask is compatible with many tumor, normal, and primary cell types often used for 3D cell culture, and may be used across many applications including:
- Drug screening
- Cancer/Tumor biology
- Stem cell biology
- Cell therapy research
- 3D tissue engineering