Recombinant Metabolic Enzymes | Recombinant Metabolic Enzyme Products | Corning

• Designed to identify potential inhibitors of cytochrome P450 activity in the early stages of drug discovery
  
• Includes all the necessary components and instructions for measuring the IC₅₀ value for up to 38 test compounds in 96-well plates. 
  
• Can accommodate other plate formats, e.g., 384 well and 1536 well, with proper scaling
  
• High stability, showing consistent IC₅₀ values and signal-to-noise ratios in tests with 10 freeze-thaw cycles.

• The use of the appropriate negative controls will eliminate the effect of possible metabolism by enzymes native to insect cells. This is required in analysis of specific metabolism of interest
• Negative controls can be used to normalize protein concentration in enzyme reaction mixture. The normalization is necessary to reduce the impact of nonspecific binding by substrates, which can result in inconsistent free concentration of substrates and hence affecting enzyme kinetics.
• Negative control microsomes and cytosols are prepared using procedures identical to those for recombinant enzyme products.

• Cofactors are necessary in many xenobiotic metabolism reactions and limitation of cofactors in a reaction will affect enzyme kinetics adversely.
• A regenerating system will produce cofactors in situ, maintaining a constant supply of cofactors for an enzymatic reaction. 
• We offer a NADPH Regenerating System for oxidase enzymes (e.g., P450, FMOs, OR), and a UDPGA Cofactor System for UGT enzymes. Each system contains 2 solutions (A and B).
• About 200 to 400 enzyme assays can be performed using each system, depending on experimental design.

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