Cryopreservation of cells and cell lines is a key technique in 3D cell culture. It preserves cells, tissues, organoids, and other biological constructs by cooling the cells to extremely cold temperatures. Maintaining cell viability through ice formation and thawing is critical.
Thanks to temperature control equipment and cryoprotective agents (CPAs), the success rate of the technique is higher than ever, according to a review published in Integrative Medicine Research.
How Does Cryopreservation Work?
Cryopreservation involves combining the cryoprotective agents (CPAs) with cells before cooling them, then freezing and storing the cells. If cells are frozen quickly, ice crystal formation, membrane damage, and osmotic shock will cause cell death. Thus, it is critical to freeze slowly. CPAs are used to affect the rate of water transport, ice crystal growth, and nucleation.
CPAs reduce the damage cells and tissues incur during the freezing and thawing process. Cells are stored in cryogenic storage containers, such as Corning® CoolCell® alcohol-free freezing containers. When the process is done correctly, the delicate structure of cells is preserved, and the cells can be thawed and remain viable.