Cell Culture Media Products | Corning

Serum has been extensively used as a supplement to chemically defined media for mammalian cell culture due to its beneficial attributes. As a complex supplement it contains proteins, growth factors, hormones, amino acids, sugars, trypsin inhibitors, and lipids which support vigorous in vitro growth of a large number of cell types. However, there are many negative aspects associated with the use of serum such as cost, time-consuming sampling, lot-to-lot variability, presence of adventitious agents, and availability. These aspects are particularly troublesome with the use of serum in the production of recombinant proteins, viral vaccines, cellular therapies, and monoclonal antibodies. In such cases, extensive downstream purification is required to remove serum-derived protein from the medium and to assure the absence of adventitious pathogens.

To minimize those problems associated with the use of serum, several defined, serum free media have been developed and commercialized. Serum-free growth media pose numerous advantages over the use of serum, including lot-to-lot consistency, reduced foaming, the reduction of exogenous agents, simpler downstream processing, and high cell yield support.

Islet cell isolation and processing for diabetes research requires a variety of specialized cell culture media, separation and gradient solutions, porcine-specific solutions, and related isolation, trimming, and storage/preservation solutions. Most islet solutions have been customized to include or leave out components such as insulin, transferrin, HSA, and HEPES.

Corning’s portfolio of specialized media has been cited in multiple islet cell isolation protocols for type 1 diabetes research. Corning’s islet solutions were designed and tailored specifically for diabetes research applications.

To achieve successful growth and consistency, media often requires nutrients, supplements, and other reagents specific to the application and cell type. Whether derived from plants or animals, or used for growing microorganisms, such as bacteria or yeast, our comprehensive line of supplements and reagents can help you create specialized culture environments and meet complex nutritional requirements for successful culture research.

Ideal for the preparation of reagents, rinsing glassware or plasticware, and other molecular biology or cell culture applications, Corning^® water can save the expense of installing and maintaining your own water system, while ensuring you have a secondary source of water that meets your specifications.

Corning water is available in a variety of sizes and tested to specifications which may include USP and/or EP monograph requirements meeting small- and large-volume demands for water to enhance molecular biology and cell culture applications.