Patented Light-tight PET Membrane
FluoroBlok cell culture inserts are designed with a patented light-tight polyethylene terephthalate (PET) membrane that efficiently blocks the transmission of light between 400 and 700 nm, allowing fluorescence detection in a simplified and non-destructive manner. Fluorescently labeled cells present in the top chamber of the insert are shielded from bottom-reading fluorescence plate readers and microscopes by the FluoroBlok membrane.
After labeled cells migrate through the membrane, they are easily detected by a bottom-reading fluorescence plate reader or microscope, thereby eliminating manual cell counting and additional processing steps. This non-destructive detection method enables both kinetic and endpoint migration and invasion assays.