Corning® NutriStem® hPSC XF Medium

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Support hiPSC and hESC growth and expansion in a feeder-free environment with NutriStem hPSC XF Medium from Corning.

overview

Enhance the single-cell culture of human induced pluripotent stem (hiPS) and human embryonic stem (hES) cells in a defined, xeno- and serum-free environment. Without the need for high levels of bFGF and other stimulatory growth factors or cytokines, low-protein NutriStem medium contains only the most essential components required for successful hiPSC and hESC maintenance, growth, and downstream differentiation.

Complete and ready-to-use, NutriStem provides a simplified medium for consistent performance, as well as increased reproducibility in hPSC long-term culture.

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NUTRISTEM IN ACTION

Superior Growth

NutriStem medium enables excellent proliferation of undifferentiated hES and hiPS cells. Proliferation of H1 hES cells cultured in Corning® Matrigel® Matrix-coated 96-well plates in NutriStem and the leading competing medium for feeder-free culture. Medium was changed and proliferation was assessed every 24 hours in culture.

Normal Colony Morphology

H1 hES cells (panel A) and ACS-1014 hiPS cells (panel B) cultured in Corning NutriStem hPSC XF Medium on Corning Matrigel matrix-coated plates display colony morphologies typical of normal feeder-free hES and hiPS cell cultures, including a uniform colony of tightly compacted cells and distinct colony edges.

Embryoid Body Formation

Embryoid bodies (EBs) were generated from H9.2 hES cells cultured for 16 passages in NutriStem hPSC XF Medium on Matrigel matrix as an evaluation of pluripotency. The pluripotent H9.2 cells were suspended in serum-supplemented medium, where they spontaneously formed EBs containing cells of embryonic germ layers. The following cell types were identified by examination of the histological sections of 14-day-old EBs stained with H&E: (A) neural rosette (ectoderm), (B) neural rosette stained with Tubulin, (C) primitive blood vessels (mesoderm), and (D) megakaryocytes (mesoderm).

Teratoma Formation

H9.2 hES cells were cultured for 11 passages in NutriStem hPSC XF Medium using a human foreskin fibroblast (HFF) feeder layer. The hES cells were subsequently injected into the hind leg muscle of SCID-beige mice for in vitro evaluation of pluripotency. The following tissues from all three germ layers were identified in H&E-stained histological sections of the teratoma 12 weeks post-injection: (A) cartilage (mesoderm), (B) epithelium (endoderm), and (C) neural rosette (ectoderm).

Long-term Culture and Single Cell Passaging of hiPSCs

Enable the expansion of hPSC cells without affecting pluripotency by combining the benefits of NutriStem medium and PureCoat rLaminin-521 vessels.

NutriStem XF Medium FAQs

From cell generation to ideal culture conditions, learn how to optimize your single-cell culture of hiPS and hES cells with NutriStem.

Application Note

NutriStem hPSC XF medium, a defined, serum-free and xeno-free medium, was used to support the long-term culture of hiPSCs on Corning Matrigel hESC-qualified matrix. Throughout a ten passage experiment, the cells remained undifferentiated, retained their pluripotency and possessed a normal karyotype.

Instructions for Use

Corning NutriStem hPSC XF medium instructions for use for the growth and expansion of human induced pluripotent stem (hiPS) and human embryonic stem (hES) cells in a feeder-free environment.

References

Corning NutriStem has been cited in a number of experiments, articles and posters with work ranging from growing methods to clinical applications for derivation and expansion of hESCs and IPSCs and much more.

Application Note

How to Buy

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Contact your authorized distributor to order NutriStem medium.

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Corning® NutriStem® hPSC XF Medium