Much has been written about scaling-up cell culture for bioprocessing and other applications that require a very high density of cells. But what about the research lab where incubators are sometimes bulging with T-175 flasks?
Although the T-175, and its other sized siblings, are tried-and-true workhorses of the cell culture bench, they offer limited options when you want to scale-up.* In most cases, researchers will increase the number of flasks they seed, which results in more handling, more processing time, and the need for more incubator space.
Which is what makes the Corning High Yielding Performance Flask (HYPERFlask) cell culture vessel such an innovative solution. Using a proprietary multilayered gas permeable growing surface to provide efficient gas exchange, Corning® HYPERFlask® vessels offer 10 times the growth area (1720 cm2) while taking up the same incubator space as a 175 cm2 flask.
Cells attach and grow on the gas permeable material with gas exchange occurring through the film layer. All fluid manipulations occur through the single neck, which is contiguous with all the layers.
In addition, Corning HYPERFlask vessels utilize the same gas permeable growing surface as Corning HYPERStack® cell culture vessels, which provide cell growth areas from 6,000 cm2 (12 stack) to 18,000 cm2 (36 stack) in a compact footprint. This makes the HYPER family a reliable scale-up path from research to bioprocessing.