Matrigel Matrix | Extracellular Matrix | Corning

Overview

Corning Matrigel Matrix

Cells behave better on Corning Matrigel matrix—the original, trusted extracellular matrix (ECM).

Nearly 30 years ago, researchers were looking for a way to grow mouse sarcoma cells. The solution? The development of Corning Matrigel matrix. Matrigel is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in such ECM proteins as laminin (a major component), collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Today, this natural ECM-based hydrogel is among the most widely used models for 2D and 3D cell culture in vitro, enabling you to:

 

  • Improve the attachment and differentiation of both normal and transformed anchorage-dependent epithelioid cells, as well as other cell types.
  • Better mimic in vivo environments for 2D and 3D cell culture applications.
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Applications

Corning Matrigel matrix is effective for the attachment and differentiation of both normal and transformed anchorage-dependent epithelioid and other cell types. These include neurons, Sertoli cells, chick lens, vascular endothelial cells, and hepatocytes.

Applications include:


  • Influencing gene expression in adult rat hepatocytes
  • 3D culture in mouse and human mammary epithelial cells
  • In vivo peripheral nerve regeneration
  • Metabolism and toxicology studies
  • The development of several types of tumor cell invasion assays
  • The in vitro and in vivo study of angiogenesis
  • In vivo propagation of human tumors in immunosuppressed mice
  • The maintenance of human embryonic stem cells and induced pluripotent stem (hiPS) cells
  • Neuronal differentiation of hiPS cells

Matrigel Matrix Quality Control Specifications

  • Mouse colonies routinely screened for pathogens via mouse antibody production (MAP) testing
  • LDEV-free EHS tumor
  • Extensive PCR testing performed on a number of pathogens, including LDEV, ensuring strict control of raw materials used during manufacturing
  • Tested and found negative for the presence of bacteria, fungi, and mycoplasma
  • Protein concentrations determined by Lowry method
  • Endotoxin units measured by Limulus Amoebocyte Lysate assay
  • Gel stability tested for a period of 14 days at 37°C
  • Biological activity determined for each lot using a neurite outgrowth assay; chick dorsal root ganglia, plated on a 1.0 mm layer of Corning Matrigel matrix, required to generate positive neurite outgrowth response after 48 hours without addition of nerve growth factor

Hear more about Dennis Plenker’s and Ping Zhou’s work and how they are using Corning® Matrigel® matrix.

Dennis Plenker

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Dennis Plenker

Ping Zhou

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Ping Zhou

Featured Resources

FAQ

Corning Matrigel Matrix FAQs

From growth factor concentration to cell recovery and storage, learn how to optimize your cell culture with Corning Matrigel matrix.

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Infographic: Why is Corning Matrigel Matrix so Popular?

Infographic: Why is Corning Matrigel Matrix so Popular?

Corning Matrigel matrix is the most highly referenced extracellular matrix and it's widely used for 2D and 3D studies, with 13,000+ citations.

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Basics of Corning® Matrigel® Matrix

Basics of Corning® Matrigel® Matrix

This webinar introduces basic information on Corning Matrigel matrix and discuss tips and techniques for handling Corning Matrigel matrix.

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Choosing the Right Cell Culture Surface: Modified Surfaces

Choosing the Right Cell Culture Surface: Modified Surfaces

In this webinar you will learn basic principles behind tissue culture treatment, and when to use enhanced attachment surfaces.

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Choosing the Right Cell Culture Surface: Extracellular Matrix Coatings

Choosing the Right Cell Culture Surface: Extracellular Matrix Coatings

In this webinar you will learn key differences between natural, mimetic, and synthetic ECM coatings and applications for specific ECM coatings.

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Why HUB Organoids are Key to Advancing Personalized Cancer Therapies

Why HUB Organoids are Key to Advancing Personalized Cancer Therapies

HUB Organoids details how patient-derived organoids are becoming promising models for personalized treatment using 3D cell culture technologies from Corning & Yamaha Corporation.

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Using Organoids in the Study of Infectious Diseases

Using Organoids in the Study of Infectious Diseases

Organoid cell culture has transformed cell-based assays in drug discovery and basic biology by conferring physiologic relevance to in vitro cell-based biological models.

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A Life Scientist’s How-To Guide on Corning Matrigel Matrix and Organoids

A Life Scientist’s How-To Guide on Corning Matrigel Matrix and Organoids

This guide details best practices to consider for organoid work, as well as how to use Corning Matrigel matrix and other products to support your organ-specific research.

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Guide to 3D Model Systems: Spheroids, Organoids and Tissue Models

Guide to 3D Model Systems: Spheroids, Organoids and Tissue Models

Download this guide to 3D models with helpful tips & techniques to set your 3D culture up for success.

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Application Notes

Tuning the Elastic Moduli of Corning® Matrigel® and Collagen I 3D Matrices by Varying the Protein Concentration

Tuning the Elastic Moduli of Corning® Matrigel® and Collagen I 3D Matrices by Varying the Protein Concentration

In this paper, we investigated the effect of protein concentration on the elastic moduli of Corning Matrigel® matrix and Collagen I gels using a rotational rheometer.

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Protocol for Corning® Matrigel® Basement Membrane Matrix for 3D Culture In Vitro

Protocol for Corning® Matrigel® Basement Membrane Matrix for 3D Culture In Vitro

Step-by-step protocol for on-top MDCK 3D culture and embedded MDCK 3D culture.

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A Novel Method for Generating Single, Intestinal Organoids for High Throughput Screening

A Novel Method for Generating Single, Intestinal Organoids for High Throughput Screening

Here we demonstrate the formation of gastrointestinal organoids derived from human induced pluripotent stem cells (hiPSC) in Corning® 96-well spheroid microplates in combination with Corning Matrigel® matrix, resulting in a single intestinal organoid in each well.

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Methods for Implantation of Corning Matrigel Matrix into Mice and Tissue Fixation

Methods for Implantation of Corning Matrigel Matrix into Mice and Tissue Fixation

Use Matrigel matrix in angiogenesis and human tumor implantation in mice.

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Maintenance of Human iPS Cells in a Feeder-free Culture System

Maintenance of Human iPS Cells in a Feeder-free Culture System

Corning Matrigel matrix is an effective surface that supports feeder-free expansion and maintenance of undifferentiated human iPS cells.

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Corning Matrigel Matrix

View All Matrigel Application Notes

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How to Buy

Purchasing Options

Order Online

Order Online

Order Online

It’s easy to purchase Matrigel matrix online. Just browse our online catalog, and add products directly to your cart. Order direct with a credit card or use a purchase order if you have an account with Corning.

It’s easy to purchase Matrigel matrix online. Order direct with a credit card or use a purchase order if you have an account with Corning.

Distributors

Distributors

Contact your authorized distributor to order Matrigel matrix.

Contact your authorized distributor to order Matrigel matrix.

Customer Service

Customer Service

If you need assistance selecting products, or if you’d like to order by phone, fax, or email, please contact us.

If you need assistance selecting products, or if you’d like to order by phone, fax, or email, please contact us.

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