Matrigel Matrix | Trusted Extracellular Matrices | Corning

Overview

Corning Matrigel Matrix

Cells behave better on Corning Matrigel matrix—the original, trusted extracellular matrix (ECM).

Nearly 30 years ago, researchers were looking for a way to grow mouse sarcoma cells. The solution? The development of Corning Matrigel matrix, a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in such ECM proteins as laminin (a major component), collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Today, this natural ECM-based hydrogel is among the most widely used models for 2D and 3D cell culture in vitro, enabling you to:


  • Improve the attachment and differentiation of both normal and transformed anchorage-dependent epithelioid cells, as well as other cell types.
  • Better mimic in vivo environments for 2D and 3D cell culture applications.
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The Secret to Choosing the Right ECM: Question Everything Free Poster Download

Question Everything Poster

Request a free poster for your lab & learn about the unquestionable choice: Corning Matrigel® matrix.

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Choosing an ECM

Corning Matrigel Matrix Products and their Applications

Tips for Using Matrigel Matrix

Top Corning Matrigel Matrix Handling Tips

How to Handle Matrigel Matrix

Learn how to store, thaw, dilute, and coat surfaces with Matrigel Matrix in your 2D and 3D cell culture applications with our easy infographic.

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Tips for Working with Corning Matrigel Matrix Aliquots

Tips for Working with Corning Matrigel Matrix Aliquots

Use this infographic for tips and tricks when working with aliquots of Matrigel matrix in organoid culture.

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FAQ

Corning Matrigel Matrix FAQs

From growth factor concentration to cell recovery and storage, learn how to optimize your cell culture with Corning Matrigel matrix.

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Basics of Corning® Matrigel® Matrix

Basics of Corning® Matrigel® Matrix

This webinar introduces basic information on Corning Matrigel matrix and discuss tips and techniques for handling Corning Matrigel matrix.

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A Life Scientist’s How-To Guide on Corning Matrigel Matrix and Organoids

A Life Scientist’s How-To Guide on Corning Matrigel Matrix and Organoids

This guide details best practices to consider for organoid work, as well as how to use Corning Matrigel matrix and other products to support your organ-specific research.

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Matrigel Matrix Case Studies

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Using Organoids for Personalized Medicine: An Interview with Sylvia Boj from HUB

Hear from Dr. Sylvia Boj, Chief Research Officer at Hubrecht Organoid Technology (HUB), to learn more about how researchers are using Corning Matrigel Matrix to generate organoid models for personalized medicine research in hopes to find the right treatment for each individual patient.

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Dennis Plenker

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Dennis Plenker

Hear more about Dennis Plenker’s work and how they are using Corning® Matrigel® matrix.  

Ping Zhou

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Ping Zhou

Hear more about Ping Zhou’s work and how they are using Corning® Matrigel® matrix.  

Matrigel Matrix Guidelines & Application Notes

Tuning the Elastic Moduli of Corning® Matrigel® and Collagen I 3D Matrices by Varying the Protein Concentration

Tuning the Elastic Moduli of Corning® Matrigel® and Collagen I 3D Matrices by Varying the Protein Concentration

In this paper, we investigated the effect of protein concentration on the elastic moduli of Corning Matrigel® matrix and Collagen I gels using a rotational rheometer.

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In Vivo Implantation

In vivo Implantation

Use Matrigel matrix in angiogenesis and human tumor implantation in mice.

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Maintenance of Human iPS Cells

Maintenance of Human iPS Cells

Matrigel for culturing human iPS cells in a feeder-free environment. 

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Protocol for Corning® Matrigel® Basement Membrane Matrix for 3D Culture In Vitro

Protocol for Corning® Matrigel® Basement Membrane Matrix for 3D Culture In Vitro

Step-by-step protocol for on-top MDCK 3D culture and embedded MDCK 3D culture.

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A Novel Method for Generating Single, Intestinal Organoids for High Throughput Screening

A Novel Method for Generating Single, Intestinal Organoids for High Throughput Screening

Here we demonstrate the formation of gastrointestinal organoids derived from human induced pluripotent stem cells (hiPSC) in Corning® 96-well spheroid microplates in combination with Corning Matrigel® matrix, resulting in a single intestinal organoid in each well.

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Methods for Implantation of Corning Matrigel Matrix into Mice and Tissue Fixation

Methods for Implantation of Corning Matrigel Matrix into Mice and Tissue Fixation

Use Matrigel matrix in angiogenesis and human tumor implantation in mice.

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Maintenance of Human iPS Cells in a Feeder-free Culture System

Maintenance of Human iPS Cells in a Feeder-free Culture System

Corning Matrigel matrix is an effective surface that supports feeder-free expansion and maintenance of undifferentiated human iPS cells.

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Corning Matrigel Matrix

View All Matrigel Application Notes

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概述

康宁Matrigel基质

细胞在康宁Matrigel基质这种原始且可靠的细胞外基质 (ECM) 上表现出良好的性能。

近30年前,研究人员一直在寻找促进小鼠肉瘤细胞生长的方法。最终的解决方案就是:康宁Matrigel基质,其为分离自Engelbreth-Holm-Swarm (EHS) 小鼠肉瘤的可溶性基底膜提取物,该肿瘤富含ECM蛋白,如层粘连蛋白 (主要成分)、IV型胶原、硫酸乙酰肝素蛋白聚糖、巢蛋白和多种生长因子。

如今,这种基于ECM的天然水凝胶是使用最广泛的体外2D和3D细胞培养模型,可使您:

  • 改善正常和转化的贴壁依赖性上皮细胞以及其他类型细胞的粘附和分化。
  • 更好地模拟体内环境,适用于2D和3D细胞培养应用。

应用

康宁Matrigel基质可有效促进正常和转化的贴壁依赖性上皮细胞以及其他类型细胞的粘附和分化,包括神经元、支持细胞、鸡晶状体、血管内皮细胞以及肝细胞

应用领域包括:

  • 影响成年大鼠肝细胞基因表达
  • 小鼠和人乳腺上皮细胞3D培养
  • 体内外周神经再生
  • 代谢和毒理学研究
  • 某些肿瘤细胞侵袭试验的开发
  • 体外和体内血管生成研究
  • 人类肿瘤在免疫抑制小鼠体内扩增
  • 人胚胎干细胞和诱导多能性干细胞 (hiPSc) 的维持
  • hiPS细胞的神经元分化 

Matrigel基质质量控制规范

  • 通过小鼠抗体产物 (MAP) 检测进行常规小鼠菌落病原体筛选
  • 不含LDEV的EHS肿瘤
  • 对包括LDEV在内的多种病原体进行广泛的PCR检测,确保对生产过程中使用的原材料进行严格控制
  • 经过检测,未发现细菌、真菌和支原体
  • 通过Lowry方法测定蛋白浓度
  • 通过鲎阿米巴样细胞溶解物试验测定内毒素水平
  • 在37°C下进行14天凝胶稳定性测试
  • 利用神经轴突生长试验测定各批次的生物活性;将鸡背根神经节接种到1.0 mm厚的康宁Matrigel基质上,48小时后,在未添加神经生长因子的情况下,神经轴突生长实验需呈阳性反应

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产品目录

产品目录

在线购买康宁产品,简单又快捷。浏览我们的线上目录获取最新产品信息。

在线购买康宁产品,简单又快捷。浏览我们的线上目录获取最新产品信息。

经销商

经销商

如需订购Matrigel基质,请联系授权经销商。

如需订购Matrigel基质,请联系授权经销商。

客户服务

客户服务

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如果需要我们帮助您选择产品,或者想要通过电话、传真或电子邮件订购,请联系我们。

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特色产品、资源及更多信息

最新信息

特色产品、资源及更多信息

康宁Matrigel基质常见问题

康宁Matrigel基质常见问题

从生长因子浓度到细胞复苏和储存,了解如何利用康宁Matrigel基质优化细胞培养效果

Corning Matrigel Matrix Frequently Asked Questions

从生长因子浓度到细胞复苏和储存,了解如何利用康宁Matrigel基质优化细胞培养效果

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康宁Matrigel批次选择和预留工具

康宁Matrigel批次选择和预留工具

确保您的实验尽可能保持一致,Matrigel基质的供应一致性完美契合您的需求

确保您的实验尽可能保持一致,Matrigel基质的供应一致性完美契合您的需求

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