Understanding the MSC Workflow: Scaling, Freezing, and Thawing
MSC cultures originate with the cells themselves, which can come directly from a patient, donor, or cryopreserved cell bank. The cells are then scaled up to the required density, depending on the application. After that, the MSCs are typically frozen with a cryoprotectant to extend the product's shelf life.
"Cryopreservation gives administrators more time for quality testing, administration, and even transportation if the site of scale-up is far from the administration site," Sherman said. "It really just expands the reach of potential therapies."
Once cells have been cryopreserved, they are thawed before being administered to patients. End to end, it's a seemingly simple sequence of scaling, freezing, and thawing, but each step of the workflow can invite new challenges.