Explore Kidney Organoids
There are several protocols used to generate kidney organoids starting from human pluripotent stem cells (hPSCs). One method was demonstrated by Dr. Melissa H. Little’s lab in 2015. Here, researchers start with hPSCs seeded on cultureware coated with Corning® Matrigel® Matrix through various induction stages to intermediate mesoderm. For 3D culture, these cells are then transferred to Transwell® Permeable Supports where they mature into kidney organoids in appropriate differentiation conditions. Read more about this research and see other kidney organoid research examples below.