Collagen for Cell Culture | Corning

Collagen for Cell Culture (2D and 3D)

Collagen for Cell Culture (2D and 3D)

Collagen is an extracellular matrix (ECM) that enables researchers to mimic in vivo environments for 2D and 3D cell culture applications through culturing a variety of cell types to promote adhesion, growth, and/or differentiation.

Improve Cell Culture Success by Promoting Adhesion, Growth, & Differentiation with Corning Collagen

Corning has developed a broad range of collagen types derived from multiple species to support your diverse needs. These high quality collagen surfaces undergo rigorous quality control testing to ensure consistent results. Corning Collagen surfaces can be applied as a thin coating, and in some cases, as a gel on a wide range of cultureware, providing the flexibility you need for diverse cell-based assays and collagen tissue engineering.

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Characteristics and Applications of Collagen

Corning® Collagen Products Frequently Asked Questions

  • Q: What does a collagen gel look like?

    A: A Corning Collagen gel looks like an agarose gel, but it is more delicate. If touched with the end  of a pipet, it will break apart

  • Q: How stable is a collagen gel over time?

    A: The Corning Collagen gel will start to contract away from the edges of a dish after 2 weeks, so we do not suggest making a gel ahead of time.

  • Q: What is the storage recommendation for collagen products?

    A: Storage recommendations for Corning Collagen products vary from 4°C to -70°C, depending on the collagen type. Specific information can be found on the product specification sheet. Frozen Collagen products should not be stored in a frost-free freezer. Avoid multiple freeze-thaws.

  • Q: What is the stability for collagen coating on a cultureware?

    A: We recommend air drying Corning Collagen-coated cultureware and then storing it at 4°C in a sealed container that will help protect it from moisture. Under these conditions the coated cultureware should be stable for at least two weeks.

  • Q: Why is collagen not gelling?

    A: Protein concentration for Collagen varies from lot-to-lot. Normalize your calculations based  on protein concentrations. It is critical the pH (e.g., for Corning Collagen I rat tail) should be as recommended in the product manual for collagen gelation. Make sure the product is stored as recommended and is not expired.

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