3D Capabilities for Automated Cell Counting of Organoids
Traditionally, Corning’s cloud-based automated cell counter has focused on rapidly capturing the detailed-analysis of 2D cell cultures. The addition of the organoid counting application expands our cell counting capabilities into the vastly developing 3D space. The robust organoid counting software package is designed to meet the unique needs of customers as 3D populations are known to vary in morphologies dependent on numerous environmental factors or protocols.
The software introduces two versions of the 3D counting algorithm:
- Version 1 (V1) is for use with cultures of irregular morphology. The machine learning algorithm for V1 is trained to engulf the individual outer membranes within the field of view in order to calculate accurate surface area and orgs./mL data.
- Version 2 (V2) of the 3D algorithm is for use with spherical objects. Other than obtaining prompt surface area and orgs./mL data; V2 will be optimal for differentiating individual spherical objects within clusters.