Please join us for this special presentation and panel discussion:
A Stimulating Panel Discussion focused on 3-Dimensional Cell Culture; Systems and Questions
Advances in HTS and failures in clinical trials have created the impetus to move towards more realistic cell-based models cultured in 3D environments. The tools and solutions used to create 3D systems have allowed researchers to assess how cells respond to stimuli in a more life-like environment. However with this progress, new challenges have arisen, including best practices on how to culture and assess 3D models in HTS systems. This panel discussion will introduce novel ways to create a 3D environment and discuss strategies and solutions to solve problems that can occur in 3D models. Bring your 3D questions and challenges for our speakers to discuss and answer as part of this interactive event.
Date: Monday, April 3, 2017
Time: 3:00 - 4:00 p.m.
Location: Spotlight Theater B – Hall A, in the Walter E. Washington Convention Center
AACR 2017 Tutorial - Panelists and Moderators
Paul Romesser, MD
Instructor of Radiation Oncology and Physician Scientist
Department of Radiation Oncology, Memorial Sloan Kettering Cancer Center
Paul Romesser, M.D., Instructor of Radiation Oncology at Memorial Sloan Kettering Cancer Center will describe his work on developing patient-derived cholangiocarcinoma and normal liver organoids as a platform to characterize and exploit genotype specific susceptibilities.
Dr. Romesser received his combined BA/MD at Boston University. He was a Howard Hughes Medical Institute research scholar at the National Cancer Institute where he studied the potential therapeutic benefit in targeting candidate oncogenes, identified in a functional genetic screen, in non-Hodgkin's lymphoma. He is currently a physician scientist in Scott Lowe's laboratory at Memorial Sloan Kettering Cancer Center.
Terry Riss, Ph.D.
Global Strategic Manager
Cell Health, Promega Corporation
Dr. Riss will discuss some potential limitations encountered when using large 3D spheroids and approaches to validate “off the shelf” assays before assuming they will work to interrogate 3D cell culture models.
Dr. Riss started the Cell Biology program at Promega Corporation in 1990 and has held several R&D and Project Management positions. Dr. Riss managed development of cell viability, cytotoxicity, apoptosis, and protease assay systems and also lead efforts to identify and promote multiplexing of cell-based assays to determine the mechanism of cell death. Dr. Riss now serves as Global Strategic Manager, Cell Health involved with selection of new areas for product development. Dr. Riss also participates in outreach educational training activities and is a co-editor of the In Vitro Cell-Based Assays section of the Assay Guidance Manual hosted by the NIH.
Hannah Gitschier, M.S.
Applications Laboratory Manager
Corning Incorporated Life Sciences
Ms. Gitschier will discuss specialty 3D cell culture techniques, including a novel model that allows for the investigation of immune cell homing, 3D tumor cytotoxicity, and tumor immune evasion, in a single, high throughput assay. The concepts presented here for immune cell interactions with 3D tumor spheroids can be extended to other co-culture models as well.
Hannah Gitschier is the Applications Lab Manager for Corning Life Sciences in Kennebunk, ME. Since joining Corning in 2011, Hannah has been part of the Applications Team focusing on developing protocols and new applications for cell culture products, cell-based and biochemical assays. Hannah received her M.S. degree in Biochemistry and Molecular Biology from the University of New Hampshire (Durham, NH), and her undergraduate degree in Biochemistry from Colgate University (Hamilton, NY).
Mark Rothenberg Ph.D.
Manager Scientific Training and Education
Corning Incorporated Life Sciences
Mark Rothenberg graduated from Emory University with his PhD in Cell and Developmental Biology. Over the past 25 years Mark has held positions in both Academia and industry where he has developed an expertise in the areas of assay development, cell culture (multiple types including primary lines), and cell culture scale-up. Prior to his current position as Manager Scientific Training and Education he worked in and was the Manager of the Applications team at Corning Life Sciences.