HepatoCells | Alternative to Primary Human Hepatocytes | Corning

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Robust Performance, Consistent Results, Easy to Use

To support the growing interest in using non-primary hepatic models for studying drug-drug interaction and cytotoxicity of xenobiotics, Corning offers a robust, consistent, and convenient solution: Corning HepatoCells (Catalog No. 354881) and companion medium (Catalog No. 354882).

HepatoCells were developed to overcome some of the shortcomings of primary human hepatocytes such as large inter-individual variability, and finite supply of high quality raw materials. These single-use, cryopreserved cells are derived from primary human hepatocytes using our proprietary immortalization technology. With a virtually unlimited supply and consistent performance, they can work as a reliable alternative in vitro hepatic model for ADME/Tox characterizations and other liver research applications. They are also ideal for long-term studies requiring large quantities of cells. Corning HepatoCells deliver:

Robust induction response for major CYP enzymes

  • Primary human hepatocyte-derived cells with primary hepatocyte characteristics
  • ≥ 10-fold induction for CYP3A4 and 1A2
     

Consistent performance

  • Low lot-to-lot variation; no need for extensive lot prequalification, a common practice with primary hepatocytes
  • High cell purity (>99% hepatocyte-like cells)
  • High post-thaw viability (≥80%) and recovery (≥8 x 106 cells per vial)
     

Convenient and easy to use
 

  • Readily available as single-use cryopreserved cells
  • Only a single medium is required for entire culturing and assay process
  • No additives, such as DMSO, required for differentiation
  • No need to use recovery or purification kits prior to plating

Characterization

A Primary-like Hepatic Model

A Primary-like Hepatic Model

Morphology: Retain Physiological Properties of Parental Hepatocytes

HepatoCells, which can be cultured up to 12 days, retain the physiological properties of their parental hepatocytes, show mature hepatocyte-like morphology, and maintain the key characteristics of primary hepatocytes, such as a robust response to CYP inducers for CYP3A4, 1A2, and 2B6. 

Image: HepatoCells cultured on 24-well Corning BioCoat™ Collagen I plates with HepatoCells culture medium. Similar morphology was observed on 48-well plates and 96-well BioCoat Collagen I plates.

Corning HepatoCells - An Alternative Hepatic Model for In Vitro ADME/Tox Applications

Phenobarbital-responsive CAR nuclear translocation, a phenomenon found in primary hepatocytes, was observed in Corning HepatoCells.  This indicates that the nuclear receptor pathways that are responsible for regulating the induction of CYP2B genes are intact in Corning HepatoCells. 

Image: EYFP-hCAR expression and localization in Corning HepatoCells and primary human hepatocytes (Data courtesy of Prof. Hongbing Wang, University of Maryland

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Request a Sample of Corning HepatoCells

Interested in trying Corning HepatoCells? Complete our short request form to get started. 

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How to Buy

Purchasing Options

Order Now

Order Now

It’s easy to purchase Corning HepatoCells online. Just browse our online catalog, and add products directly to your cart. Order direct with a credit card or use a purchase order if you have an account with Corning.

It’s easy to purchase Corning HepatoCells online. Just browse our online catalog, and add products directly to your cart. Order direct with a credit card or use a purchase order with Corning Account.

Distributors

Distributors

Corning HepatoCells and companion medium is sold directly in the U.S. For global customers outside of U.S., please contact your regional Corning office.

Corning HepatoCells and companion medium is sold directly in the U.S. For global customers outside of U.S., please contact your regional Corning office.

Customer Service

Customer Service

If you need assistance selecting products, or if you’d like to order by phone, fax, or email, please contact us.

If you need assistance selecting products, or if you’d like to order by phone, fax, or email, please contact us.

Applications and Use

HepatoCells in Action

HepatoCells in Action

Corning HepatoCells User Guide

For specific information on best practices, this HepatoCells User Guide details the process, materials to be used, the protocol for thawing cyropreserved HepatoCells, and a protocol for performing a CYP450 induction assay.

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Corning HepatoCells: An In Vitro Screening Tool for Predicting Clinical CYP3A4 Induction

HepatoCells were evaluated for their applicability as an in vitro screening tool for predicting clinical CYP3A4 inducers. Three lots of cells were treated with a group of known clinical strong, moderate/weak inducers, and non-inducers at more than 8 concentrations each. Results showed that all three lots of HepatoCells behaved similarly to primary human hepatocytes in terms of prediction, with only minor lot-to-lot variations for the 3 lots of cells.

 

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Corning HepatoCells form Cell Spheroids, Enabling 3D Microtissue Assays

Corning 96-well Ultra-Low Attachment spheroid microplates were used to culture
HepatoCells into single spheroids at indicated plating cell density (100mL/well). As shown in these photos taken on day 7, HepatoCells can form spheroids with cell number dependent size.

 

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Corning HepatoCells Closely Model the Behavior of Parental Cells for Predicting Hepatotoxicity

The performance of HepatoCells is compared to the parental hepatocyte cells and other hepatic models in order to determine if these cryopreserved cells model the behavior of parental primary human hepatocytes following in vitro exposure to known hepatotoxic compounds. Results suggest that HepatoCells closely model the functional behavior of parental cells and therefore may be used as a primary hepatocyte surrogate to study pharmacogenomic-related cytotoxicity.

 

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