October 16 and 17, 2018 | Sheraton Hotel | Boston, MA

Ready to Rock the Science of 3D

3D cell culture is exploding. To capture the energy and excitement of this moment in research, Corning is bringing together industry leaders and innovators for a 3D Cell Culture User Summit. The event will be a forum to share ideas and information, discuss the future of 3D applications, and to network with key influencers in the space.

Bringing your Cells to the Third Dimension | A Hands-on Workshop

October 16, 2018 | 1:00 – 4:30 p.m. 

Benefit from a half day hands-on workshop working with scientists from Corning and other leading life sciences companies to help fine tune your 3D cell culture models. The workshop will address 3D cell culture techniques, including advanced and novel models. 3D methods discussed will include the use and analysis of research that includes Corning spheroid microplates, Transwell® permeable supports, and extracellular matrices, including Corning Matrigel® matrix. The workshop will conclude with a Q&A session with a panel of experts.

By attending this workshop you’ll learn: 

  • 3D cell culture and handling methods
  • Novel and advanced 3D approaches and systems
  • Tips and techniques for 3D high-content imaging
  • Current and trending applications for 3D cell culture models

Space is limited, reserve your spot now >>

From Promise to Reality in your Lab: Practical 3D Tools and Applications

The workshop will address 3D cell culture techniques, including advanced and novel models. 3D methods discussed will include the use and analysis of research that includes Corning spheroid microplates, Transwell® permeable supports, and extracellular matrices, including Corning Matrigel® matrix. We will discuss some of the unique applications exclusive to these technologies including immune oncology models, blood brain barrier models, and organoid culture.

3D Assay Optimization

Building upon the earlier presentation on 3D tools and applications, we will delve into tips and techniques that will support 3D optimization across various types of 3D cell culture models and assays.

Considerations and Approaches for the Complete 3D Characterization of 3D Cell Culture Models Using High Content Confocal Microscopy

Over the last ten years 3D cell culture models (e.g., organoids, spheroids, microtissues) have been rapidly adopted by researchers looking to improve the in vivo relevant of their in vitro models. However, one of the major problems with 3D cell culture models is characterization wherein imaging-based approaches can severely bias results as they only characterize cells on the periphery of these models where they are most exposed to compounds and nutrients. This sampling bias can result in inaccurate results from studies and loses the spatial information that makes these models intrinsically more valuable than 2D cell culture models. In this presentation we will describe approaches to characterizing every cell within a 3D cell culture model through the use of confocal imaging and considerations for labeling, imaging, and image processing.

Cell Health Determinations in 3D-Cultures: Challenges and Opportunities

A majority of plate-based cell health assay chemistries were originally developed for use with 2D monolayers.  The size, geometry, and structural complexity of 3D cultures often present barriers to directly measuring biomarkers associated with changes in cell health or proliferation.  This talk will present basic strategies and factors to consider for successfully applying and validating cell health reagents for 3D culture models.  Protocol modifications and purpose-fit alternative approaches for specific cell health parameters associated with viability, cytotoxicity and apoptosis will be discussed.

3D Cell Culture Summit

October 17, 2018 | 8:00 a.m. – 5:00 p.m. 

Join Corning for our full day 3D Cell Culture Summit to:

  • Hear from top pharmaceutical and academic researchers on topics like HTS with spheroids, organoid-based models and 3D bioprinting
  • Network with peers and industry thought leaders
  • Learn about what’s new and next from Corning Research and Development

Space is limited, reserve your spot now >>

Meeting Objectives

  • Connect industry leading researchers and thought leaders to discuss 3D applications using Corning products
  • Introduce new users to 3D best practices and novel techniques to get started in 3D cell culture
  • Networking and discussion among industry leaders on 3D workflow optimization and new ideas

Questions? Contact us.

Active-Learning Strategies for High Content Screening of 3D Tumor Cell Models

The Discovery Biology Automation team at GSK has designed an intelligent, automated platform to accelerate the identification of high value small molecule leads and the delivery of quality candidates into clinical development. To achieve human pharmacology for discovery leads, the team is engaging in multidisciplinary collaborations to develop disease-relevant in vitro preclinical models to improve clinical relevance. Alejandro will introduce novel 3D tumor in vitro models, high content imaging and cell-based screening technologies that improve clinical relevance in the testing of oncology drugs.

Cerebral Organoids as a 3D Model for Glioma

Current preclinical models of glioblastoma multiforme (GBM) are limited by the lack of a “normal” human microenvironment and the inability to recapitulate key biological features of the human disease. To address these limitations, we have established a unique GBM model system using human embryonic stem cell (hESC)-derived cerebral organoids and patient-derived glioma stem cells (GSCs). Using our cerebral organoid glioma model, we demonstrate a powerful tool for investigating GBM biology within a human brain microenvironment and for modeling diverse therapeutic interventions.

3D Organoids Reveal a Critical Role for Microenvironment in Polycystic Kidney Disease

Kidney organoids are three-dimensional units with proximal tubules, distal tubules, and podocytes in patterned segments, recapitulating the architectural subunit of the kidney. Using CRISPR-Cas gene editing, we have further generated kidney organoids with loss-of-function mutations in genes associated with polycystic kidney disease (PKD), which form cysts from kidney tubules similar to the human disease. When PKD organoids are grown in the absence of adherent cues, the rate of cystogenesis is greatly increased, and the organoids expand 4,000-fold in size, generating cysts that can readily be seen in by eye. Screening of organoids in high throughput formats further identifies a new role for non-muscle myosin in this disease, which may facilitate attachments between tubular cells and their surroundings. These findings reveal a critical role for microenvironment in human PKD, and raise new possibilities for understanding disease from the cellular to the organoid scale.

Bioprinted Three-Dimensional Human Tissues for Disease Modeling

Successful prediction of candidate drugs can be hampered by the limitations of in vitro tools to model complexities of human tissue biology. This translational challenge can result in low safety and efficacy predictability and contribute to attrition in drug development. To bridge this gap, the Organovo NovoGen® Bioprinting Platform has been utilized to develop ExVive™ 3D Bioprinted Tissues, fully cellular 3D tissue models fabricated by automated spatially controlled cellular deposition. The multicellular architecture of the 3D model can better recapitulate native tissue structure and function compared to standard in vitro models, allowing for complex, tissue-level phenotypes associated with chronic injury and disease.

In vitro Disease Models for Screening: from 3D Differentiation to Potential Drug Discovery

Our laboratory is interested in the study of neurodegenerative and neuropsychiatric disorders using human pluripotent stem cell-derived neurons. Using spinner flasks and 3D differentiation protocols, we can generate different types of neurons (motor neurons, cortical neurons, dopaminergic neurons) and glial cells (astrocytes). The large-scale production of cells allows us to use small molecule screening to simultaneously interrogate the molecular mechanisms underlying a target of interest as well as potentially opening new avenues for drug discovery. One example of our work is a recent screen for small molecule modulators of the complement component 4 (C4) system in stem cell-derived human astrocytes. With this approach, we have identified different classes of compounds that decrease the amount of C4 in vitro as well as show the efficacy of one of them in vivo.

Cancer Modeling with Three-Dimensional Organoids

Cancer is a major cause of death, and development of new cancer therapies requires in vitro and in vivo models that reproduce key features of human disease. This presentation will summarize key recent developments in cancer research using three-dimensional organoids. Work from our lab highlights the application of CRISPR-Cas9 gene editing to study colorectal cancer biology in organoid systems.

Predicting Drug Response with Primary Cell Derived Spheroids

KIYATEC has developed a wide array of 3D tissue models to answer a variety of drug response questions in vitro. These models range from simple spheroid static cultures to far more complex multi-cell type perfusion cultures with the unifying factor being a focus on the utilization of primary cells from cancer patients. In this talk I will discuss our rationale of model selection, describe the pros and cons of spheroids, and provide evidence of the ability to accurately predict response ex vivo using this platform.

What’s New and Next in 3D from Corning

Description: Coming soon!

More information: Coming soon!