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可能性はあります。浸潤率を算出して補正することをお勧めします。
<計算式>
%浸潤の計算式:
%浸潤= | (マトリゲルインサートメンブレンを浸潤している平均細胞数)/(コント ロールインサートメンブレンを移動する平均細胞数)×100 |
浸潤指数の計算式:
浸潤指数= | (%浸潤試験細胞)/(%浸潤コントロール細胞) |
詳しくは、ユーザーズガイドを参照してください。
プロトコール(英語)(44.4KB)
プロトコール(日本語)(470KB)
参考文献
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4. Duxbury, M.A., Ito, H., Zinner, M.J., Ashley, S.W., Whang, E.E. EphA2: a determinant of malignant cellular behavior and a potential therapeutic target in pancreatic adenocarcinoma. Oncogene. 23:1448 (2004).
5. Seton-Regers, S.E., Lu, Y., Hines, L.M., Koundinya, M., LaBaer, J., Muthuswamy, S.K., Brugge, J.S. Cooperation of the ErbB2 receptor and transforming growth factor in induction of migration and invasion in mammary epithelial cells. Proc. Natl. Acad. Sci. 101(5):1257 (2004).
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7. Takada, Y., Kobayashi, Y., Aggarwal, B.B. Evodiamine abolishes constitutive and inducible NF-κB activation by inhibiting IκBα kinase activation, thereby suppressing NF-κB-regulated antiapoptotic and metastatic gene expression, up-regulating apoptosis, and inhibiting invasion. J. Biol. Chem. 280(17):17203 (2005).
8. Ichikawa, H., Takada, Y., Murakami, A., Aggarwal, B.B. Identification of a novel blocker of I kappa B alpha kinase that enhances cellular apoptosis and inhibits cellular invasion through suppression of NFkappa B-regulated gene products. J. Immunol. 174(11):7383 (2005).
9. Silvera, D., Rezina, A., Darvishian, F., Levine, P.H., Zolfaghari, L., Goldberg, J., Hochman, T., Formenti, S.C., Schneider, R.J. Essential role for eIF4GI overexpression in the pathogenesis of inflammatory breast cancer. Nat. Cell Biol. 11:903-908 (2009).
10. Kajiro, M., Hirota, R., Nakajima, Y., Kawanowa, K., So-ma, K., Ito, I., Yamaguchi, Y., Ohie, S., Kobayashi, Y., Seino, Y., Kawano, M., Kawabe, Y., Takei, H., Hayashi, S., Kurosumi, M., Murayama, A., Kimura, K., Yanagisawa, J. The ubiquitin ligase CHIP acts as an upstream regulator of oncogenic pathways Nat. Cell Biol. 11:312-319 (2009).