Corning® Cell Culture Surfaces:
Poly-D-lysine Coated Surface
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Figure 1. The amino group on the end of each lysine develops a net positive charge in culture medium which makes it hydrophilic and a good site for cell attachment.
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Some assays and procedures require enhanced binding of cells to polystyrene. Corning poly-D-lysine (PDL) microplates are coated with PDL by a proprietary method. This synthetic polymeric coating creates a uniform net positive charge on the plastic surface which, for some cell types, can enhance cell attachment, growth and differentiation, especially in serum-free and low serum conditions.
PDL surfaces often improve attachment and growth of primary neurons, glial cells, neuroblastomas, and a variety of transfected cell lines, including HEK-293 (see Figure 1). PDL surfaces are often used to reduce cell detachment that often occurs during washing steps in cell-based assays.
Other Cell Culture Surfaces
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Available Products (Catalog)
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