Corning® Cell Culture Surfaces:
Ultra-Low Attachment Surface

  C6 Colony on ULA C6 cell colony
  Figure 1. C6 glioma cell colonies derived from single cells form unattached spherical colonies (spheroids) on the Ultra-Low Attachment surface on tissue (left panel) but grow as flattened attached colonies in standard tissue culture treated surface (right panel).

The Corning Ultra-Low Attachment surface is a covalently bound hydrogel layer that is hydrophilic and neutrally charged. Since proteins and other biomolecules passively adsorb to polystyrene surfaces through either hydrophobic or ionic interactions, this hydrogel surface naturally inhibits nonspecific immobilization via these forces, thus inhibiting subsequent cell attachment. This surface is very stable, noncytotoxic, biologically inert and nondegradable.

The Ultra-Low Attachment surface is useful for:

  • Growing primary cultures of tumor or normal cells as unattached spheroids
  • Studying activation and inactivation mechanisms of lymphocytes, monocytes, macrophages and other phagocytic cells
  • Research and procedures requiring the reduction of eukaryotic or microbial cell attachment
  • Preventing anchorage-dependent cells, such as fibroblasts, from attaching and dividing
     Embryoid Body on ULA
     Figure 2. Corning Ultra-Low Attachment surface inhibits differentiation and promotes tight well-defined embryoid body formation in murine ES cell grown in methylcellulose medium for 3 days.
  • Selectively culturing tumor or transformed cells as unattached colonies (or a substitute for soft agar assays (Figure 1)
  • Culturing cells, tissue or organ fragments in a stationary suspended, unattached state
  • Tissue engineering of easily harvested cell sheets and constructs using magnetic nanoparticles or liposomes

It is ideal for culturing stem cells:

  • Promotes embryoid body formation (Figure 2)
  • Gives colonies with defined margins
  • Cleaner morphology
  • Makes colonies easier to harvest


Other Cell Culture Surfaces

Helpful Information

 Untreated Polystyrene Surface
 Standard Tissue Culture Treated Surface
 Corning® CellBIND® Surface
 Poly-D-lysine Surface
 Synthemax™ Surface
 Corning® Osteo Assay Surface

 Cell Culture Surface References
 Ultra-Low Attachment Surface
 Cell Culture Surface Selection Chart
 Cell Culture Surfaces Document Library
 Request a Sample


Available Products (Catalog)

 All Ultra-Low Attachment Vessel Products