Corning® Assay Surfaces:
Sulfhydryl-BIND™ (Maleimide) Modified Surface
| |
|
| |
Figure 1. Reaction of maleimide with biomolecule containing a sulfhydryl group on the Sulfhydryl-BIND™ surface allows site-directed molecular orientation.
|
Corning's Sulfhydryl-BIND™ surface has covalently-linked maleimide groups that covalently couple to sulfhydryl groups (via SH moieties) and are ideal for assays requiring site-directed orientation of a particular biomolecule, especially antibodies (Figure 1). Minimal surface preparation is required: sulfhydryl groups need to be made accessible by the reduction of disulfide bonds or by modification with 2-iminothiolane-HCl (Traut's reagent).
Sulfhydryl-BIND Surface Properties
|
|
Applications
|
Assays requiring site-directed orientation of the biomolecule. Useful for the retention or optimization of activity - enzymatic, immunological, etc.
|
|
# Reactive Sites
|
1 x 1014/cm2
|
|
Binding Interaction
|
Covalent site-specific
|
|
Sample Properties
|
Must have an available sulfhydryl group for covalent coupling via SH moieties
|
|
Surface/Sample Preparation
|
Sulfhydryl groups need to be made accessible by the reduction of disulfide bonds or by modification with 2-Iminothiolane-HCI. Diluent: pH 6-6.5
|
|
Blocking Feasibilty & Method
|
Difficult. Block with 2% BSA in sodium phosphate buffer. Add 10% FBS to antibody diluent.
|
|
Performance Criteria
|
Must demonstrate specific binding: preference for SH, modified goat IgG-Fc over non-modified goat IgG-Fc
|
Other Microplate Surfaces
| | Helpful Information
|
| | |
| |
Available Products (Catalog)
|
|
| |