Corning® Assay Surfaces:
Medium Binding (Untreated) Polystyrene Surface
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Figure 1. Untreated polystyrene is naturally hydrophobic and is a medium binding surface.
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Natural, unmodified polystyrene surfaces are hydrophobic in nature and can only bind biomolecules through passive interactions (Figure 1). This type of surface is referred to as medium binding and is primarily suitable for the immobilization of large molecules, such as antibodies, which have large hydrophobic regions that can interact with the surface. Due to the large surface area needed to immobilize biomolecules in this manner, binding capacities are typically low: 100-200ng IgG/cm2.
Medium Binding Polystyrene Surface Properties
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Applications
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EIA and RIA
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# Reactive Sites
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Binding capacity: 250 ng lgG/cm². 1cm²= 100 µl vol.
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Binding Interaction
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Hydrophobic |
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Sample Properties
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High molecular weight: >20kD. Large or abundant hydrophobic regions. Proteins, glycoproteins, lipoproteins, polysaccharides.
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Surface/Sample Preparation
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None
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Blocking Feasibilty & Method
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Easy. Block with 0.02% Tween 20 or 1% BSA
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Performance Criteria
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Well to well CV < 5%. Averge high and low wells from the mean <15%.
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* Note: 500 ng lgG/cm2 is equivalent to monolayer coverage, which should not be exceeded if reproducible results are desired. An observed increase in covalent binding capacity as compared to passive adsorption is due to optimal orientation of the molecule on the surface leading to increased reactivity.
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Other Microplate Surfaces
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Available Products (Catalog)
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