Corning® Assay Surfaces:
DNA-BIND® (N-oxysuccinimide) Modified Surface
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Figure 1. Reaction of N-oxysuccinimide (NOS) with an aminated biomolecule makes the DNA-BIND® surface ideal for immobilizing DNA and other primary amine containing molecules.
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Corning's DNA-BIND® surface has covalently linked N-oxysuccinimide esters (referred to as NOS groups) that react with nucleophiles such as primary amines at slightly alkaline pH. The NOS ester undergoes nucleophilic substitution as the amine attacks the carbonyl group and displaces the N-oxysuccinimide group (Figure 1). The resulting amine linkage covalently couples primary amine containing molecules to the surface of DNA-BIND® microplates.
While this surface was specifically designed for the immobilization of aminated DNA for use in nucleic acid hybridization assays and solid-phase PCR, peptides and other small primary amine containing molecules can also be coupled to the surface. This amine reactive chemistry (with approximately 1 x 1014/cm2 reactive sites) enables the development of a wide variety of assays for high-throughput screening. This surface is not recommended for binding large proteins.
DNA-BIND Surface Properties
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Applications
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Immobilization of small antigens (single amino acids), aminated single stranded oligonucleotides. Not suitable for the immobilization of antibodies. Black and white plates for fluorescence and luminescence applications. DNA-BIND Thermowell M PCR plates can be used in solid phase applications.
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# Reactive Sites
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1 x 1014/cm2
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Binding Interaction
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Covalent peptide bonds
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Sample Properties
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Must have an amine group available for covalent coupling.
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Surface/Sample Preparation
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No preparation required. Diluent: pH 8-9
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Blocking Feasibilty & Method
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Difficult. Block with 2% BSA in sodium phosphate buffer. Add 10% FBS to antibody diluent.
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Performance Criteria
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Must demonstrate covalent binding of alkaline phosphatase as compared to passive adsorption
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Other Microplate Surfaces
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Available Products (Catalog)
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