Corning® Assay Surfaces:
Aminated Polystyrene Surface
Polystyrene can also be modified to possess positively charged amine groups that replace the benzene ring at the surface (Figures 1 and 2). This type of surface lacks hydrophobic character and is strictly ionic in nature. Using the appropriate buffers and pH, this surface can be used to ionically couple to small negatively charged biomolecules. The lack of hydrophobic areas on the surface precludes the immobilization of large, relatively hydrophobic molecules. This surface is used with bifunctional crosslinkers (i.e., glutaraldehyde, carbodiimide) to covalently couple to functional groups (primary amines, thiols, and carboxyls) on biomolecules. Due to its hydrophilic nature and capability of covalent immobilization through the use of additional crosslinkers, this surface can be used to immobilize molecules solubilized in detergents, such as Triton® X-100 and Tween 20.
|
|
|
Figure 1. Here the benzene groups have been replace with amine groups to create an aminated polystyrene surface.
|
| |
|
| |
Figure 2. Immobilization to aminated surfaces is a two-step process.
|
Aminated Surface Properties
|
|
Applications
|
Assays requiring solid phase covalent immobilization of reactants.
|
|
# Reactive Sites
|
2 x 1013/cm2
|
|
Binding Interaction
|
Covalent via bifunctional crosslinkers
|
|
Sample Properties
|
Must possess an amine, carboxyl, sulfhydryl or other appropriate functional group available for covalent coupling.
|
|
Surface/Sample Preparation
|
Surface must be activated with a bifunctional crosslinker, such as a monomeric gluteraldehyde, bis-sulfosuccinimidyl suderate or carbodiimide.
|
|
Blocking Feasibilty & Method
|
Difficult: Block with 1 to 2% Non Fat Dry Milk. Alternative blocking reagents must possess an appropriate fuctional group.
|
|
Performance Criteria
|
Must demonstrate covalent immobilization in the presence of stergents. Covalent activity equal to or greater than 75%; CV less than or equal to 5%.
|
Other Microplate Surfaces
| | Helpful Information
|
| | |
| |
Available Products (Catalog)
|
|
| |