Assay Surfaces
The Chart below will help you find the best Corning polystyrene surfaces for the binding or covalent immobilization of cells, proteins, nucleic acids, and other types of biomolecules for use in microplate-based assays that do not require cell attachment. For information on Corning microplate surfaces for cell-based assays see Cell Culture Surfaces. Click on the desired surface for additional technical information.
Corning Surface
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Binding Interaction
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Binding Properties1
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NBSTM surface
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None - Inhibits hydrophobic and ionic interactions
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Significantly reduces (<2 ng/cm2) protein and nucleic acid binding
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Medium Binding (Untreated) surface
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Hydrophobic
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Large biomolecules >20kD with large or abundant hydrophobic regions
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High Binding suface |
Hydrophobic and ionic (negatively charged)
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Improves binding of medium to large biomolecules (>10kD) that are positively charged with or without hydrophobic regions |
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Aminated Surface
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Hydrophilic and ionic (positively charged); allows covalent immobilization
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Small negatively charged biomolecules OR biomolecules possessing an appropriate functional amine, carboxyl or thiol group.
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DNA-BIND® surface |
Allows covalent immobilization to amine groups via binding to NOS groups
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Small to medium biomolecules, especially DNA, possessing an available amine group.
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Sulfhydryl-BINDTM surface
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Allows covalent immobilization via SH moietics on maleimide groups
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Biomolecules possessing an accessible sulfhydryl group or reducible disulfide bond.
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Carbo-BINDTM surface
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Allows covalent immobilization via binding to hydrazide groups
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Biomolecules possessing carbohydrate moieties available for periodate activation.
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Universal-BINDTM surface
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Allows covalent immobilization via UV cross-linking to abstractable hydrogen
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Biomolecules with abstractable hydrogen. |
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1Choosing the appropriate assay surface requires that the structure of the molecule to be immobilized be known, so that functional groups available on the molecule can be matched with the correct surface. Of particular concern is that the functional group on the biomolecule interacting with the surface must be positioned in such a manner that immobilization does not interfere with its immunological or enzymatic activity.
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