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Corning® Microarray Surfaces:
Corning® GAPS™ II Coated Slides

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	Figure 1. GAPS™ II Coated Slides have a positive charge to facilitate ionic interactions with oligonucleotides and proteins.

Corning® GAPS™ II Coated Slides have a uniform, covalently bound coating of Gamma Amino Propyl Silane (GAPS; Figure 1) on both sides.

The GAPS coating is applied using a proprietary process under tightly controlled manufacturing conditions resulting in a uniform substrate of unmatched cleanliness and high binding capacity. The GAPS™ II Coated Slides offer a robust, yet economical, substrate to researchers engaged in the fabrication of arrays of double-stranded DNA and arrays of soluble proteins (Figure 2).

For additional technical information, check the GAPS™ II Coated Slides Instruction Manual. 

Figure 2. The Caspase-3 substrate NH2-DEVDA-Biotin was suspended in Corning® Epoxide Spotting Solution and printed in quadruplicate onto anhydride-derivatized GAPS™ II Coated Slides. Peptide arrays were incubated with Avidin-Cy®3 in the absence or presence of Caspase-8 or Caspase-3 (as indicated), and scanned at 532 nm. The printed DEVDA peptide retained function on the array, as indicated by the reduced fluorescence seen in the spots treated with Caspase-3, but not Caspase-8. GAPS™ II Coated Slides have also been used successfully for protein arraying without derivatization.