Use of Label-Free Optical Biosensors to Detect Ion Channel Interactions in Intact Cells

     Presented By Professor L. K. Kaczmareck, Ph.D.
     Department of Pharmacology, Yale University School of Medicine

     Tuesday, July 7, 2009
     10:00 am - 11:00 am EST or 16:00 - 17:00 (UK); 17:00 - 18:00 (Europe)

Based on optical biosensor technology, the label-free Corning Epic^® System is sensitive enough to detect ion channel activity by monitoring the dynamic mass redistribution of intracellular proteins. Traditional assays examining the interaction between channels and regulatory cytoplasmic proteins generally provide no information on the time course of interactions in living cells. This presentation will describe the use of the Epic System to probe the interactions of Slack sodium-activated potassium channels with potassium channel openers and with cytoplasmic constituents. The Epic System is a high-throughput, label-free detection platform with demonstrated utility for drug discovery in both biochemical and cell-based assay applications.

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Grow More Cells With Corning: Scaling Up Cell Production in Research Laboratories

     Presented By Vitaly Klimovich, Ph.D.

     Tuesday, July 21, 2009
     12 noon - 1 pm EST or 17:00 - 18:00 (UK); 18:00 - 19:00 (Europe)

     Thursday, July 23, 2009
     9:00 am - 10:00 am EST or 14:00 - 15:00 (UK); 15:00 - 16:00 (Europe)

Life science researchers are constantly being asked to grow more cells for cell-based experiments and assays, and producing recombinant proteins, antibodies, vaccines and viral vectors. This live, on-line seminar will help you select the vessels and techniques that can best meet your needs for growing large amounts of cells (up to 1010 cells) or producing more products from these cells in a typical research laboratory setting. One goal of this seminar is to keep the scale up process as simple as possible and thus increase the likelihood of success. Another goal is to save money or reduce costs and make the scale up process more affordable to research laboratories faced with tight budgets.

Topics covered will include:

• Advantages and disadvantages of the different vessels and systems used for growing large amounts of cells;
• How to select the best culture system for your needs
• How to increase your chances of success

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Cell-Culture Contamination: Every Researchers Nightmare, Part I

     Presented By Mark Rothenberg, Ph.D.
    
     Tuesday, September 8, 2009
     12 noon - 1 pm EST or 17:00 - 18:00 (UK); 18:00 - 19:00 (Europe)

     Thursday, September 10, 2009
     9:00 am - 10:00 am EST or 14:00 - 15:00 (UK); 15:00 - 16:00 (Europe)

Recent studies have shown that at least 20% of the animal cell cultures currently in use in the US are contaminated by either microorganisms or other cell lines! This three part live, on-line seminar series will review the scope of this major problem and examine some of its causes and techniques for avoiding it. It will also explore some key, easy to employ strategies for preventing these losses by careful culture management.

Part I: Understanding Culture Contamination

• Understanding the nature of biological and chemical culture contaminants, especially mycoplasma –today’s most serious and widespread culture contaminant;
• Consequences of contamination;
• Understanding the major sources and causes of cell culture contamination.

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Cell-Culture Contamination: Every Researchers Nightmare, Part II

     Presented By John So, M.S.

     Tuesday, September 22, 2009
     12 noon - 1 pm EST or 17:00 - 18:00 (UK); 18:00 - 19:00 (Europe)

     Thursday, September 24, 2009
     9:00 am - 10:00 am EST or 14:00 - 15:00 (UK); 15:00 - 16:00 (Europe)

Recent studies have shown that at least 20% of the animal cell cultures currently in use in the US are contaminated by either microorganisms or other cell lines! This three part live, on-line seminar series will review the scope of this major problem and examine some of its causes and techniques for avoiding it. It will also explore some key, easy to employ strategies for preventing these losses by careful culture management.

Part II: Good Aseptic Technique

• Developing a practical approach to aseptic technique;
• Reducing day-to-day contamination problems in the lab;
• Helpful hints for avoiding contamination

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Cell-Culture Contamination: Every Researchers Nightmare, Part III

     Presented By Jeanne Phillips, Ph.D.

     Tuesday, October 6, 2009
     12 noon - 1 pm EST or 17:00 - 18:00 (UK); 18:00 - 19:00 (Europe)

     Thursday, October 8, 2009
     9:00 am - 10:00 am EST or 14:00 - 15:00 (UK); 15:00 - 16:00 (Europe)

Recent studies have shown that at least 20% of the animal cell cultures currently in use in the US are contaminated by either microorganisms or other cell lines! This three part live, on-line seminar series will review the scope of this major problem and examine some of its causes and techniques for avoiding it. It will also explore some key, easy to employ strategies for preventing these losses by careful culture management.

Part III: Managing Culture Contamination - Strategies for managing and reducing cell culture contamination long term

• Reducing cell culture accidents;
• The hidden dangers of using antibiotics in culture;
• Simple and practical contamination monitoring methods;
• Strategic use of cryopreserved cultures

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"We are not getting information like this from any where. The seminar was amazing, very useful to my work. Thanks for organizing these sessions."

"Great tips! I'll pass along information I learned here to my colleagues to let them know how we should conduct cell culture properly. Many thanks indeed again."

"I use your seminars as training for new employees and estimate they save my company more than $24,000 a year in training costs."

"I have been doing cell culture for 20+ years, and found the presentation informative and thought provoking. One can never learn enough about cell culture as it is an evolving art/science."

Founded in 1925, ATCC is the world's leading Biological Resource Center. Its mission is to acquire, authenticate, preserve, produce, develop and share biological materials for the advancement of scientific knowledge. ATCC offers over 3,600 cell lines from 80 species as well as fully tested media, sera and tissue culture reagents for growing them. Its mission is to acquire, authenticate, preserve, develop and distribute biological materials, information, technology, intellectual property and standards for the advancement, validation and application of scientific knowledge.