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Corning® Microarray Surfaces
Corning offers a choice of three optimized surfaces for creating microarrays as
well as two printing solutions and two hybridization solutions further
optimized for these surfaces (Table 1).
Corning offers three surfaces
for printing microarray slides |
Table 1. Corning® Surfaces for Printing Microarrays
| Slide Surface
|
Recommended Printed Content
|
Binding Interaction
|
Recommended Printing Solutions
|
Recommended Hybridization Solutions
|
| Corning®
Epoxide Coated Slides |
cDNA, long oligonucleotides (>50-mer)
|
Covalent |
Pronto!™ Epoxide Spotting Solution |
Pronto!™ cDNA/Long Oligo Hybridization
Solution
|
| Short oligonucleotides (~30-mer) with or
without amino modifications |
Covalent |
Pronto!™ Epoxide Spotting Solution |
Pronto!™ Short Oligo Hybridization
Solution |
| UltraGAPS™
Coated Slides |
cDNA, long oligonucleotides (>50-mer) |
Ionic |
Pronto!™ Universal Spotting Solution |
Pronto!™ cDNA/Long Oligo Hybridization
Solution |
| GAPS™
II Coated Slides |
cDNA, long oligonucleotides (>50-mer) |
Ionic |
Pronto!™ Universal Spotting Solution |
Pronto!™ cDNA/Long Oligo Hybridization
Solution |
| Proteins |
Ionic |
Content-dependent |
Ligand-dependent |
All Corning® Microarray Slides are manufactured under the most stringent
conditions, cleaned and individually examined for mechanical defects and the
presence of dust and glass particles. Surfaces are applied in an
environmentally controlled, HEPA-filtered ISO Class 5 facility, resulting in
coated slides with highly uniform surface properties and low autofluorescence.
Surface wettability is consistent across the slide surface to assure uniform
spot size and shape and to avoid uncontrolled wicking or poor volume transfer
during printing. Packaging has been developed to maintain the appropriate
storage environment.
