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Corning® Cell Culture Surfaces:
Corning® CellBIND® Polystyrene Surface

Find out how you could win a share of $100,000 of Corning products by using this innovative product

The unique Corning CellBIND surface is the first novel cell culture surface treatment in over 20 years. This new surface enhances attachment making it easier to grow fastidious cells under difficult conditions, such as reduced-serum or serum-free medium, resulting in higher cell yields.

Developed by Corning scientists, this patented (US Patent # 6,617,152) technology uses a microwave process for treating the culture surface. This new process improves cell attachment by incorporating significantly more oxygen into the cell culture surface, rendering it more hydrophilic and increasing surface stability.

Treatment	Oxygen incorporation (by ESCA)	Contact Angle1 (Lower is more wettable)
Corning CellBIND Surface	29.0%	12.3° – 16.3°
Traditional Tissue Culture Surface	17.2%	55.8° – 63.5°
Competitor Amine Surface	14.1%	74.9°
1Vessels were incubated for 5 days at 52°C.

Electron Spectroscopy Chemical Analysis (ESCA) shows that the amount of oxygen incorporated into the Corning CellBIND surface is over 60% higher than with the traditional tissue culture surface treatment. Click here for more information on Corning CellBIND® surface.

Some customer cell lines that have performed better on the Corning CellBIND Surface:
CHSE-214	HUVEC
PC-12	LNCaP
HEK-293	3T3
CHO	HEK-293
HTB-13	Caco-2
Calu-3	Hep-G2
Saos-2	U-2 OS
MBDK	16HBE
E4 embryo	DLD1
2/4/A1	HCT-116
A549	C8B4
1° Prostate	Melanoma
Preadipocytes	1° Rat liver
Hi-5 (BTI-Tn-5B1-4)
Endothelial cells
1° mouse embryo fibroblasts

Unlike poly-D-lysine and biological coatings, the Corning CellBIND surface is a nonbiological surface that requires no special handling or storage. Because the polymer is treated, rather than coated, the surface is consistent and stable. This enhanced performance has already led to a major biotechnology company choosing Corning roller bottles with the Corning CellBIND surface for producing a new FDA approved protein therapeutic.

Corning CellBIND Surface Benefits

• Quickly adapts cells to reduced serum or serum-free conditions
• May eliminate the need for expensive coatings
• Improves attachment and yield
• No special handling or storage required

Corning CellBIND Surface works with:

• Primary Cultures – helps them attach without need for expensive biological coatings
• Roller bottle cultures – cells attach faster and remain attached longer
• Transfected cells – helps them stay attached when over-expressing proteins
• Cell-based assays – reduces cell losses during washing steps for lower CVs

(Click here to find out why ATCC® recommends this surfae for PC-12 cell.)

Learn more about what products are now available with the Corning CellBIND Surface in our Product Catalog

• Dishes
• Flasks
• 6, 12, 24, 48, 96 and 384 Well Microplates
• CellSTACK® Culture Chambers
• Roller Bottles
• 100-Layer CellCube® Module
• 25-Layer CellCube® Module
• RoboFlask™ Vessels
• HYPERFlask® Vessels

Technical Information

• Adherent Growth of CHO cells Thermo Scientific HyClone SFM4CHO-A medium using Corning® CellBIND® Surface Culture Flasks - Application Note
• Instrument and Microplate Considerations to Improve Image Capture and Data Generation During High Content Screens - Application Note
• Corning CellBIND Surface: An Improved Surface for Enhanced Cell Attachment - Technical Report
• Reducing Serum Levels and Culture Costs - Application Note
• Enhanced Attachment of LNCaP Cells to the Corning CellBIND Surface - SnAPPShots
• Enhanced Survival of LNCaP Cells Following Cryopreservation Using the CryoStor™ CS5 Preservation Solution and Corning® CellBIND® Surface - SnAPPShots
• The Synergistic Effect of Serum-Free Medium with the Corning CellBIND Surface on HEK 293 Cells - SnAPPShots

Product Literature

• Corning CellBIND® Surface: A novel surface for improved cell attachment

References

Request a sample

NOTE: Most samples will be shipped upon the request standard UPS ground service; however some products require additional qualification prior to the request being fulfilled. For these items a Corning Life Sciences Account Manager will contact you with additional information.