Corning® Assay Surfaces:
DNA-BIND® (N-oxysuccinimide) Modified Surface

Corning's DNA-BIND® surface has covalently linked N-oxysuccinimide esters (referred to as NOS groups) that react with nucleophiles such as primary amines at slightly alkaline pH. The NOS ester undergoes nucleophilic substitution as the amine attacks the carbonyl group and displaces the N-oxysuccinimide group (Figure 1). The resulting amine linkage covalently couples primary amine containing molecules to the surface of DNA-BIND® microplates. While this surface was specifically designed for the immobilization of aminated DNA for use in nucleic acid hybridization assays and solid-phase PCR, peptides and other small primary amine containing molecules can also be coupled to the surface. This amine reactive chemistry (with approximately 1 x 10¹⁴/cm² reactive sites) enables the development of a wide variety of assays for high-throughput screening. This surface is not recommended for binding large proteins.

Figure 1. Reaction of N-oxysuccinimide (NOS) with an aminated biomolecule makes the DNA-BIND® surface ideal for immobilizing DNA and other primary amine containing molecules.

Product Information

• 96 Well Microplates

Technical Information