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Oligonucleotides (~30-mers) were printed onto a Corning Epoxide or a
competitor’s
covalent slide following recommended
protocols. Oligos were either C6-amine
modified at the 5’ end (A columns) or
unmodified (B columns). Cy5/Cy3 ratios
correlated strongly between modified and unmodified oligos for Corning Epoxide
slides, but the competitor’s slide showed
an absolute requirement for amino modification.
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Corning® Epoxide Coated Slides and Pronto!™
Universal Microarray Reagents and Kits
Corning® Epoxide Coated Slides are the latest generation of high
performance microarray slides. Designed for covalent attachment of modified or
unmodified short oligos (~30-mers), but they also work with long oligos and
cDNA – now one slide can meet all your microarray needs. For optimum
performance use with Corning’s specially formulated Epoxide Spotting Solution
Spotting solution to obtain small, consistent spot sizes allowing for very high
density arrays. The Pronto!™ Universal Hybridization Kit has been
optimized for the precise specificity and stringency required for hybridizing
labeled cDNA to short oligo microarrays.
The advantages are easy to see:
-
Slides can be used to attach short oligos, long oligos and cDNA – now one slide
for all your microarray needs.
-
Interslide CVs of 10% or less gives consistent results for comparison of data
between experiments and between slides.
-
Direct labeling of less than 5µg total RNA saves valuable samples and reduces
overall costs.
-
Covalent attachment of DNA to slide reduces number of steps involved in process
because no UV cross-linking or baking is necessary.
Learn more about Corning® Epoxide Coated Slides and Pronto!™ Universal
Microarray Kits and Reagents in our Product Catalog
