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Cultrex® BME coated 8µm pore polycarbonate Transwell® inserts for 24 well plates with BME are ready to use for cell invasion assays.

Transwell® Permeable Supports Coated with Cultrex® Basement Membrane Extract

New 24 Well Transwell inserts aseptically precoated with Cultrex® Basement Membrane Extract (BME) by Trevigen, Inc. are now available with 8.0 µm pore polycarbonate membranes. Cultrex Basement Membrane Extract is a soluble form of basement membrane purified from Engelbreth-Holm-Swarm (EHS) tumor. The major components of the BME include laminin I, collagen IV, entactin, and heparin sulfate proteoglycan.

  • Ideal for cell invasion assays and evaluating immune responses, wound healing and angiogenesis
  • Helps measure cell migration in vitro in response to chemoattractants and/or inhibiting compounds.
  • Aseptically coated with BME purified from Engelbreth-Holm-Swarm (EHS) tumors
  • 12 precoated inserts per 24 well plate, 2 plates per case
  • Cell migration can be measured using Calcein AM or other staining techniques. (See Instructions for Use.)
  • Currently available only in the United States

Click here to learn more about Cultrex® coated Transwell® Inserts in our Product Catalog

Technical Literature

HT1080 and MCF7 Cell Invasion Assay Performance


Comparison of noninvasive MCF7 cells and invasive HT1080 cells in a standard cell invasion assay using 8.0 µm pore Transwell inserts precoated with Cultrex Basement Membrane Extract in response to the chemoattractant FBS.


Reference

Fridman, R., Kibbey, M.C., Royce, L.S., Zain, M., Sweeny, T.M., Jicha, D.L., Yannelli, J.R., Martin, G.R., Kleinman, H.K. 1991. Enhanced Tumor Growth of Both Primary and Established Human and Murine Tumor Cells in Athymic Mice after Co-injection with Matrigel. Journal of the National Cancer Institute. 83:769-774.


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