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Corning® Cell Culture Surfaces:
Standard Tissue Culture Treated Polystyrene Surface

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	Figure 1. Corning's treatment modifies the surface by adding oxygen containing chemical groups or opening the benzene ring (not shown).

Standard Corning® polystyrene cell culture vessels are surface modified using either corona discharge (flasks, dishes and microplates) or gas-plasma (roller bottles and culture tubes). These processes generate highly energetic oxygen ions which graft onto the surface polystyrene chains (Figure 1) so that the surface becomes hydrophilic and negatively charged when medium is added (Hudis, 1974; Amstein and Hartman, 1975; Ramsey et. al., 1984; see References ). The more oxygen that is incorporated on to the surface the more hydrophilic it becomes and the better it is for cell attachment and spreading.

This standard Corning cell culture surface:

• Has been successfully used to grow cells for over 30 years
• Works with most attachment-dependent cell lines
• Is very economical