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Corning Cell Culture Surfaces:
Corning® Osteo Assay Surface

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	Figure 1. TRAP positive multinucleated osteoclasts differentiated from rat primary cells (left panel). Resorption pits formed by functional osteoclasts (right panel).

The Corning Osteo Assay surface is an inorganic crystalline calcium phosphate coating on a polystyrene plate designed to mimic in vivo bone environment. The surface is intended to facilitate a variety of bone cell assays including immunofluorescent staining, tartrate resistant acid phosphatase (TRAP), and surface resorption assays for osteoclasts. The surface is available in a convenient, ready to use, sterile, multiple well plate and is stable at room temperature.

The Osteo Assay surface is useful for:

Figure 2. Corning Osteo Assay surface promotes co-culture of metastatic breast cancer cell line, MDA MB231(small light pink stained cells)  and multinucleated osteoclast (dark pink)

• Cell growth and differentiation of precursor cells to osteoclast or blast
• Direct readout of osteoclast and osteoblast  cell function and differentiation assays such as TRAP staining, pit resorption and bone nodule formation
• Targeted drug screening for bone cell activity
• Real time imaging for high content analysis 
• Quantitative assay for enzymatic activity by sampling the cell culture supernatant

Ideal for Bone and Metastatic Cells Co-cultures (Figure 2)