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Corning® Synthemax Surface

  hesc_morph_174_161 hesc_undiff_174_161
  Figure 1. H7 Human Embryonic Stem Cell (hESC) morphology. hESC cultured in chemically defined medium on the Synthemax surface 6 well plate (left panel, 25X phase contrast). Oct4 protein expression demonstrates pluripotency (right panel, 25X immunofluorescence).
The Corning Synthemax surface is designed to mimic a cells' natural environment with extra cellular matrix derived cell adhesion promoting peptides. This surface allows expansion and differentiation of embryonic stem cells for more than 20 serial passages in defined xeno-free medium with:  
  • Stable doubling time and cell viability
  • Retention of phenotypic marker expression such as Oct4, TRA-1-60, SSEA4 and cell morphology (Figure 1)
  • Retention of normal karyotype
  • Retention of pluripotency as determined by embryoid body formation and teratomas
  • Directed differentiation into specific tissue types such as cardiomyocytes (Figure 2)

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 Figure 2. Cardiomyocytes differentiated from H7 hESC on Corning Synthemax Surface.  Cells stained with Cardiomyocyte specific markers alpha-actinin (green), Nkx2.5 (red) and nuclear marker DAPI (blue).

Synthemax Surface Characteristics Ideal for GMP Compliant Cell Production Facilities:

  • Synthetic, xeno-free substrate
  • Gamma sterilized (SAL 10-6)
  • Quality tested for lot-to-lot consistency and traceability
  • Stored at room temperature for 2 years
  • Ready to use
  • Available in multiple formats: 6 well plates and T-75 flasks


 

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Available Products (Catalog)


 Synthemax Surface Products