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Corning® Synthemax Surface

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	Figure 1. H7 Human Embryonic Stem Cell (hESC) morphology. hESC cultured in chemically defined medium on the Synthemax surface 6 well plate (left panel, 25X phase contrast). Oct4 protein expression demonstrates pluripotency (right panel, 25X immunofluorescence).

The Corning Synthemax surface is designed to mimic a cells' natural environment with extra cellular matrix derived cell adhesion promoting peptides. This surface allows expansion and differentiation of embryonic stem cells for more than 20 serial passages in defined xeno-free medium with:  

• Stable doubling time and cell viability
• Retention of phenotypic marker expression such as Oct4, TRA-1-60, SSEA4 and cell morphology (Figure 1)
• Retention of normal karyotype
• Retention of pluripotency as determined by embryoid body formation and teratomas
• Directed differentiation into specific tissue types such as cardiomyocytes (Figure 2)

	Figure 2. Cardiomyocytes differentiated from H7 hESC on Corning Synthemax Surface.  Cells stained with Cardiomyocyte specific markers alpha-actinin (green), Nkx2.5 (red) and nuclear marker DAPI (blue).

Synthemax Surface Characteristics Ideal for GMP Compliant Cell Production Facilities:

• Synthetic, xeno-free substrate
• Gamma sterilized (SAL 10^-6)
• Quality tested for lot-to-lot consistency and traceability
• Stored at room temperature for 2 years
• Ready to use
• Available in multiple formats: 6 well plates and T-75 flasks

 

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