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Corning® Assay Surfaces:
Aminated Polystyrene Surface

Polystyrene can also be modified to possess positively charged amine groups that replace the benzene ring at the surface (Figures 1 and 2). This type of surface lacks hydrophobic character and is strictly ionic in nature. Using the appropriate buffers and pH, this surface can be used to ionically couple to small negatively charged biomolecules. The lack of hydrophobic areas on the surface precludes the immobilization of large, relatively hydrophobic molecules. This surface is used with bifunctional crosslinkers (i.e., glutaraldehyde, carbodiimide) to covalently couple to functional groups (primary amines, thiols, and carboxyls) on biomolecules. Due to its hydrophilic nature and capability of covalent immobilization through the use of additional crosslinkers, this surface can be used to immobilize molecules solubilized in detergents, such as Triton® X-100 and Tween 20.

Aminated Surface

Figure 1.  Here the benzene groups have been replace with amine groups to create an aminated polystyrene surface.

  Immobilization to Aminated Surface
  Figure 2. Immobilization to aminated surfaces is a two-step process.

 

 

 

 

 

 

 

 

 

 

 

 

Aminated Surface Properties 

Applications Assays requiring solid phase covalent immobilization of reactants.
# Reactive Sites 2 x 1013/cm2 
Binding Interaction  Covalent via bifunctional crosslinkers
Sample Properties Must possess an amine, carboxyl, sulfhydryl or other appropriate functional group available for covalent coupling.
Surface/Sample Preparation Surface must be activated with a bifunctional crosslinker, such as a monomeric gluteraldehyde, bis-sulfosuccinimidyl suberate or carbodiimide.
Blocking Feasibilty & Method    Difficult: Block with 1 to 2% Non Fat Dry Milk. Alternative blocking reagents must possess an appropriate fuctional group.
Performance Criteria  Must demonstrate covalent immobilization in the presence of stergents.  Covalent activity equal to or greater than 75%; CV less than or equal to 5%.

Other Microplate Surfaces


Helpful Information


 NBS™ Coated Polystyrene
 Medium Binding (Untreated) Polystyrene
 High Binding Modified Polystyrene
 DNA-BIND® Modified Polystyrene
 Sulfhydryl-BIND™ Modified Polystyrene
 Carbo-BIND™ Modified Polystyrene
 Universal-BIND™ Modified Polystyrene

 Assay Surface References
 Assay Surfaces Document Library
 Microplate Surface Selection Chart
 Equipment Compatibility Guide
 Request a Sample

 

Available Products (Catalog)


 All Aminated Modified Polystyrene Products