featured product: Corning® Nonbinding Surface (NBS™) Microplates



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Corning® Nonbinding Surface (NBS™) Microplates
Improving assay sensitivity and performance

Highlights

Key Features and Benefits

Comparison of protein and nucleic acid binding with various polymers

Frequently Asked Questions

NBS™ Product Listing

Sample Request

NBS Scientific Seminar (May 20, 2004)

Product Literature

Technical Information

Product Catalog – 96 Well NBS™ Microplates

Product Catalog - 384 Well NBS™ Microplates

Highlights

Cornin® NBS™ microplates improve assay performance for high-throughput screening applications.

Reduce nonspecific binding for increased assay sensitivity

Enhance signal-to-noise ratio at low concentrations

Save on reagent costs

Ideal for homogeneous and SPA assays

Available in 96 and 384 well formats

Automation-friendly microplates can be bar coded

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Key Features and Benefits of Corning® NBS™ Microplates

Features

Benefits

Nonbinding surface

•	Reduces nonspecific binding of expensive reagents to microplate surface

•	Improves signal and reduces background for homogeneous and SPA assays

Thermally stable from 4° to 37°C

•	Consistent performance over a wide range of temperature and storage conditions

Chemically stable noncytotoxic surface

•	Compatible with aqueous solutions with low levels (<20%) of organic solvents such as ethanol and DMSO

Meets SBS microplate standards

•	Compatible with standard 96 and 384 well liquid handling and automation instrument

•	Suitable for bar coding

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Comparison of protein and nucleic acid binding with various polymers

	Binding in ng/cm2
	¹²⁵I-IgG	¹²⁵I-BSA	¹²⁵I-Insulin	³²P-oligo DNA	³²P-l phage DNA
Polystyrene (PS)	400	450	310	22	6
Polypropylene 380	380	440	370	3	<2
NBS on PS	<2.5	<2.5	5	<2	<2
Based on a Scintillation Proximity Assay (SPA) using 100 µL/well in 96 well microplates. Contents were aspirated and washed 3 times with 200 µL/well of PBS, pH 7.4 (Ref. 1).

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Frequently Asked Questions

What is Corning® NBS™ surface?
NBS™ is a Corning proprietary surface technology that is based on a high molecular weight polyethylene oxide-containing polymer that is insoluble in water.

How stable is the surface to repeated use with aqueous solutions?
NBS™ is very stable to aqueous solutions, except those with high levels (>20%) of organic solvents (e.g., DMSO, ethanol).

Why do some customers prefer to use NBS™ microplates instead of untreated microplates?
Some customers using “sticky” proteins in assays reported significant protein losses to the plate surface. By switching to the Corning® NBS™ microplates, they saw a notable improvement in assay signal and sensitivity as well as maintenance of activity over a period of time. Other customers had developed a low volume assay and could only detect acceptable signals with the NBS™ microplates. The added benefit for them was the decreased reagent usage, which ultimately led to lower assay costs.

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NBS™ Product Listing

Ordering Information

Corning® NBS™ microplates are available in 96 and 384 well formats. These microplates are nonsterile and have a flat bottom, unless otherwise indicated.

Cat. No.	Description	Qty/Pack	Qty/Case
96 Well NBS Microplates
3641	96 well, clear solid plate	25	100
3650	96 well, black solid plate	25	100
3991	96 well, black solid plate	5	25
3686	96 well, black solid half area plate	25	100
3993	96 well, black solid half area plate	5	25
3651	96 well, black with clear bottom plate	25	100
3881	96 well, black with clear bottom half area plate	25	100
3600	96 well, white solid plate	25	100
3990	96 well, white solid plate	5	25
3605	96 well, white solid plate, round bottom	25	100
3642	96 well, white solid half area plate	25	100
3992	96 well, white solid half area plate	5	25
3604	96 well, white with clear bottom plate	25	100
3995	96 well, white with clear bottom plate	5	25
3884	96 well, white with clear bottom half area plate	25	100
3994	96 well, white with clear bottom half area plate	5	25
384 Well NBS Microplates
3654	384 well, black solid plate	25	100
3676	384 well, black solid low volume plate, round bottom	25	100
3655	384 well, black with clear bottom plate	25	100
3640	384 well, clear solid plate	25	100
3652	384 well, white solid	25	100
3653	384 well, white clear bottom	25	100
3673	384 well, white solid low volume plate, round bottom	25	100
For additional product or technical information, please visit www.corning.com/ lifesciences or call 1.800.492.1110. Outside the United States, please call 1.978.635.2200.

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