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Corning® Cell Culture Surfaces:
Poly-D-lysine Surface


Poly-D-Lysine Surface

  Figure 1. The amino group on the end of each lysine develops a net positive charge in culture medium which makes it hydrophilic and a good site for cell attachment.

Some assays and procedures require enhanced binding of cells to polystyrene. Corning poly-D-lysine (PDL) microplates are coated with PDL  by a proprietary method. This synthetic polymeric coating creates a uniform net positive charge on the plastic surface which, for some cell types, can enhance cell attachment, growth and differentiation, especially in serum-free and low serum conditions.

PDL surfaces often improve attachment and growth of primary neurons, glial cells, neuroblastomas, and a variety of transfected cell lines, including HEK-293 (see Figure 1). PDL surfaces are often used to reduce cell detachment that often occurs during washing steps in cell-based assays.

Other Cell Culture Surfaces

Helpful Information

 Untreated Polystyrene Surface
 Ultra-Low Attachment Surface
 Standard Tissue Culture Treated Surface
 Corning® CellBIND® Surface
 Corning® Osteo Assay Surface
 Corning® Synthemax Surface

 Poly-D Lysine Surface
 Cell Culture Surface References
 Cell Culture Surfaces Document Library
 Cell Culture Surface Selection Chart
 Request a Sample


Available Products (Catalog)

 96 Well Microplates
 384 Well Microplates