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Corning® Assay Surfaces:
Universal-BIND™ Modified Surface

The photo reactive Universal-BIND™ surface is intended to covalently immobilize biomolecules via abstractable hydrogen using UV illumination resulting in a carbon-carbon bond. All that is required is a UV crosslinker; no surface preparation or pre-activation is necessary. Although the linkage is nonspecific and does not allow for site-directed orientation of a biomolecule, this surface is very useful for the following applications:

  • Immobilization of double-stranded DNA
  • Immobilization of antigens of unknown structure (available functional groups unidentified)
  • Immobilization of samples containing a mixture of biomolecules, such as cell lysate samples
  • Immobilization of other nonproteinaceous molecules, such as glycolipids

Universal-BIND Surface Properties 

Applications Immobilization of double-stranded DNA (*See note below), antigens of unknown structure (available functional groups unidentified), samples containing a mixture of biomolecules.
# Reactive Sites 1 x 1014/cm2
Binding Interaction  Covalent non-specific via abstractable hydrogens 
Sample Properties Double-stranded DNA (*See note below), antigens of unknown structure, cell lysate samples, glycolipids.
Surface/Sample Preparation Biomolecules need to be immobilized via UV crosslinking.
Blocking Feasibilty & Method    Difficult. Block with 2% BSA in sodium phosphate buffer. Add 10% FBS in antibody diluent.
Performance Criteria  Must demonstrate covalent immobilization when UV crosslinked as compared to non-UV crosslinked.
*Note: dsDNA cannot be denatured once bound to this surface; both strands are covalently attached.

Other Microplate Surfaces


Helpful Information


 NBS™ Coated Polystyrene
 Medium Binding (Untreated) Polystyrene
 High Binding Modified Polystyrene
 Aminated Modified Polystyrene
 DNA-BIND® Modified Polystyrene
 Sulfhydryl-BIND™ Modified Polystyrene
 Carbo-BIND™ Modified Polystyrene

 Assay Surface References
 Assay Surfaces Document Library
 Microplate Surface Selection Chart
 Equipment Compatibility Guide
 Request a Sample

 

Available Products (Catalog)


 All Universal-BIND™ Modified Polystyrene Products