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Assay Surfaces

The Chart below will help you find the best Corning polystyrene surfaces for the binding or covalent immobilization of cells, proteins, nucleic acids, and other types of biomolecules for use in microplate-based assays that do not require cell attachment. For information on Corning microplate surfaces for cell-based assays see Cell Culture Surfaces. Click on the desired surface for additional technical information.

Corning Surface

Binding Interaction

Binding Properties1

NBSTM coated polystyrene surface    None - Inhibits hydrophobic and ionic interactions Significantly reduces (<2 ng/cm2) protein and nucleic acid binding
Medium Binding (Untreated) modified polystyrene surface   Hydrophobic  Large biomolecules >20kD with large or abundant hydrophobic regions 
High Binding modified polystyrene suface   Hydrophobic and ionic (negatively charged)  Improves binding of medium to large biomolecules (>10kD) that are positively charged with or without hydrophobic regions 
Aminated modified polystyrene surface   Hydrophilic and ionic (positively charged); allows covalent immobilization  Small negatively charged biomolecules OR biomolecules possessing an appropriate functional amine, carboxyl or thiol group.
DNA-BIND® modified polystyrene surface       Allows covalent immobilization to amine groups via binding to NOS groups Small to medium biomolecules, especially DNA, possessing an available amine group. 
Sulfhydryl-BINDTM modified polystyrene surface  Allows covalent immobilization via SH moietics on maleimide groups  Biomolecules possessing an accessible sulfhydryl group or reducible disulfide bond. 
Carbo-BINDTM modified polystyrene surface    Allows covalent immobilization via binding to hydrazide groups  Biomolecules possessing carbohydrate moieties available for periodate activation. 
Universal-BINDTM modified polystyrene surface  Allows covalent immobilization via UV cross-linking to abstractable hydrogen  Biomolecules with abstractable hydrogen. 
1Choosing the appropriate assay surface requires that the structure of the molecule to be immobilized be known, so that functional groups available on the molecule can be matched with the correct surface. Of particular concern is that the functional group on the biomolecule interacting with the surface must be positioned in such a manner that immobilization does not interfere with its immunological or enzymatic activity.

 

Helpful Information 


 All Assay Surfaces Product Catalog
 Assay Surface References
 Assay Surfaces Document Library
 Microplate Surface Selection Chart
 Equipment Compatibility Guide
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