Corning® Assay Surfaces:
Universal-BIND™ Modified Surface
The photo reactive Universal-BIND™ surface is intended to covalently immobilize biomolecules via abstractable hydrogen using UV illumination resulting in a carbon-carbon bond. All that is required is a UV crosslinker; no surface preparation or pre-activation is necessary. Although the linkage is nonspecific and does not allow for site-directed orientation of a biomolecule, this surface is very useful for the following applications:
- Immobilization of double-stranded DNA
- Immobilization of antigens of unknown structure (available functional groups unidentified)
- Immobilization of samples containing a mixture of biomolecules, such as cell lysate samples
- Immobilization of other nonproteinaceous molecules, such as glycolipids
Universal-BIND Surface Properties
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Applications
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Immobilization of double-stranded DNA (*See note below), antigens of unknown structure (available functional groups unidentified), samples containing a mixture of biomolecules.
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# Reactive Sites
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1 x 1014/cm2
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Binding Interaction
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Covalent non-specific via abstractable hydrogens
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Sample Properties
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Double-stranded DNA (*See note below), antigens of unknown structure, cell lysate samples, glycolipids.
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Surface/Sample Preparation
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Biomolecules need to be immobilized via UV crosslinking.
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Blocking Feasibilty & Method
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Difficult. Block with 2% BSA in sodium phosphate buffer. Add 10% FBS in antibody diluent.
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Performance Criteria
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Must demonstrate covalent immobilization when UV crosslinked as compared to non-UV crosslinked.
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*Note: dsDNA cannot be denatured once bound to this surface; both strands are covalently attached.
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Other Microplate Surfaces
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Available Products (Catalog)
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