Corning® Assay Surfaces:
Carbo-BIND™ (Hydrazide) Modified Surface
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Figure 1. Reaction of hydrazide with a periodate-activated glycosylated biomolecule on the Carbo-BIND™ surface allows site-directed molecular orientation. |
Corning's Carbo-BIND™ modified surfaces have hydrazide groups that covalently couple to carbohydrate (CHO) groups, and are ideal for assays requiring site-directed orientation of a particular biomolecule (oxidized antibodies, carbohydrates and glycosylated proteins) while maintaining enzymatic or immunological activity (Figure 1).
Minimal surface preparation is required: the target molecule needs to be oxidized by the addition of sodium periodate or other appropriate methods.
Carbo-BIND Surface Properties
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Applications
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Assays requiring site-directed orientation of the biomolecule. Useful for the retention or optimization of activity - enzymatic, immunological, etc.
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# Reactive Sites
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1 x 1014/cm2
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Binding Interaction
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Covalent site-specific via CHO moieties
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Sample Properties
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Oxidized antibodies, periodate activated carbohydrates or glycosylated biomolecules.
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Surface/Sample Preparation
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The biomolecule needs to be oxidized by the addition of Na-periodate or other appropriate methods.
Diluent: pH 5-5.5
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Blocking Feasibilty & Method
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Medium. Block with 2% BSA in Tris buffer
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Performance Criteria
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Must demonstrate specific binding: preference for periodate-activated alkaline phosphatase over non-activated alkaline phosphatase. |
Other Microplate Surfaces
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Available Products (Catalog)
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